Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-7-10
pubmed:abstractText
In animal cells, most microtubules are nucleated at centrosomes. At the onset of mitosis, centrosomes undergo a structural reorganization, termed maturation, which leads to increased microtubule nucleation activity. Centrosome maturation is regulated by several kinases, including Polo-like kinase 1 (Plk1). Here, we identify a centrosomal Plk1 substrate, termed Nlp (ninein-like protein), whose properties suggest an important role in microtubule organization. Nlp interacts with two components of the gamma-tubulin ring complex and stimulates microtubule nucleation. Plk1 phosphorylates Nlp and disrupts both its centrosome association and its gamma-tubulin interaction. Overexpression of an Nlp mutant lacking Plk1 phosphorylation sites severely disturbs mitotic spindle formation. We propose that Nlp plays an important role in microtubule organization during interphase, and that the activation of Plk1 at the onset of mitosis triggers the displacement of Nlp from the centrosome, allowing the establishment of a mitotic scaffold with enhanced microtubule nucleation activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1534-5807
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-25
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:12852856-Animals, pubmed-meshheading:12852856-Cell Cycle Proteins, pubmed-meshheading:12852856-Cell Line, pubmed-meshheading:12852856-Centrosome, pubmed-meshheading:12852856-Glutathione Transferase, pubmed-meshheading:12852856-Humans, pubmed-meshheading:12852856-Microtubule-Associated Proteins, pubmed-meshheading:12852856-Microtubules, pubmed-meshheading:12852856-Models, Biological, pubmed-meshheading:12852856-Mutation, pubmed-meshheading:12852856-Nuclear Proteins, pubmed-meshheading:12852856-Phosphorylation, pubmed-meshheading:12852856-Protein Kinases, pubmed-meshheading:12852856-Protein-Serine-Threonine Kinases, pubmed-meshheading:12852856-Proto-Oncogene Proteins, pubmed-meshheading:12852856-Recombinant Proteins, pubmed-meshheading:12852856-Substrate Specificity, pubmed-meshheading:12852856-Tubulin, pubmed-meshheading:12852856-Xenopus
pubmed:year
2003
pubmed:articleTitle
Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation.
pubmed:affiliation
Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152, Martinsried, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't