Linked Life Data

1285196

Source:http://linkedlifedata.com/resource/pubmed/id/1285196

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-12-27
pubmed:abstractText
A cytoplasmic translation inhibitory ribonucleoprotein (iRNP), about 10-12S in size and containing a heterogeneous class of RNA (iRNA) in the 60-140 nucleotide size range, has been previously isolated from 13-14 day old chick embryonic leg and breast muscle. Both iRNA and iRNA are potent inhibitors of mRNA translation in vitro. A cDNA library for iRNA was prepared in pUC18 vector. The 114 nucleotide insert of one clone was subcloned in pBluescript vector. The in vitro transcript of this insert (only the 5'-3' orientation and not the reverse orientation) showed inhibition of muscle poly(A)+ mRNA translation in vitro. The derived sequence of the iRNA indicates that it is a new sequence in which 27 nucleotides at the 3' and 5' ends can form base paired stem. Hybridization experiments and sequence analysis indicate that this iRNA has complementary (antisense) sequence to a highly conserved domain of the 28S eukaryotic rRNA and to a 5' leader segment of a muscle mRNA. These results suggest that iRNA mediated translational inhibition may act as a novel mechanism of controlling cellular mRNA levels in embryonic muscle and thus, acts in conjunction with the transcriptional control of myogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0081-1386
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
363-71
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
The cytoplasmic translational inhibitory RNA of chick embryonic muscle: cloning and sequence of a subspecies which is antisense RNA.
pubmed:affiliation
Department of Anatomy and Cellular Biology, Tufts University School of Veterinary Medicine, Boston, Massachusetts 02111.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't