Source:http://linkedlifedata.com/resource/pubmed/id/12849740
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2003-7-9
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pubmed:abstractText |
The hyperpolarization-activated h channel current (Ih) reported to be present in acutely isolated rat dorsal root ganglion (DRG) neurons is inhibited by Cs+ and ZD7288. It was recently reported that lead (Pb2+) inhibits voltage-gated Ca2+ and K+ channels in DRG neurons but the effect of Pb2+ on Ih has so far not been reported. Using whole-cell patch clamp technique we show that Pb2+ specifically inhibited Ih. External application of 0.1, 1 and 10 microM Pb2+ reversibly reduced the amplitude of Ih in a dose-dependent manner, with an IC50 value of 3.7 microM and a Hill coefficient of 1.1. Pb2+ shifted the activation curve of Ih by 9.3 mV but had no effect on the slope factor. Pb2+ inhibited Ih in a voltage-dependent manner and slowed down the activation process, indicating an action of Pb2+ on the activation kinetics of h channels. Our studies thus demonstrated that Pb2+ is a dose-dependent, voltage-dependent and reversible blocker of Ih in DRG neurons.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic Nucleotide-Gated Cation...,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Lead,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/hyperpolarization-activated cation...
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pubmed:status |
MEDLINE
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pubmed:issn |
0306-4522
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
57-63
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12849740-Animals,
pubmed-meshheading:12849740-Cyclic Nucleotide-Gated Cation Channels,
pubmed-meshheading:12849740-Dose-Response Relationship, Drug,
pubmed-meshheading:12849740-Ganglia, Spinal,
pubmed-meshheading:12849740-Ion Channels,
pubmed-meshheading:12849740-Lead,
pubmed-meshheading:12849740-Membrane Potentials,
pubmed-meshheading:12849740-Neural Inhibition,
pubmed-meshheading:12849740-Neurons,
pubmed-meshheading:12849740-Potassium Channels,
pubmed-meshheading:12849740-Rats,
pubmed-meshheading:12849740-Rats, Wistar
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pubmed:year |
2003
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pubmed:articleTitle |
Pb2+ inhibits the hyperpolarization-activated current in acutely isolated dorsal root ganglion neurons.
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pubmed:affiliation |
Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada. xdai@ualberta.ca
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pubmed:publicationType |
Journal Article
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