Linked Life Data

12848483

Source:http://linkedlifedata.com/resource/pubmed/id/12848483

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2003-7-9
pubmed:abstractText
The speciation of selenium compounds from high selenium broccoli (876 microg/g) depends on the extraction conditions. Twenty-seven extraction conditions were explored involving nine different buffering systems between pH 1 and pH 9. In nonbuffered extractions of broccoli, more than 40% of the spiked Se-methylselenocysteine was not recovered in the filtered solution. However, in buffered extractions, losses for Se-methylselenocysteine ranged from 10 to 20%. Mass balance indicated that approximately 30% of naturally occurring selenium in broccoli samples was volatilized and lost to the atmosphere when buffered extractions were made. Solid phase extractions indicated that the polarity of selenium compounds in solution was also dependent on the extracting solution. High-pressure liquid chromatography coupled to an inductively coupled plasma mass spectrometer was used to show that selenium compounds extracted from broccoli reacted with the extracting solution. Compound identities were assigned by matching retention times to standards of selenite, selenate, methylseleninic acid, Se-methylselenocysteine, selenomethionine, and the selenonic acids of Se-methylselenocysteine and selenomethionine. Changes in speciation were analyte-, pH-, and buffer-dependent, but generally, a higher pH resulted in more highly oxidized selenium compounds. For valid conclusions to be drawn from the analytical data, the extraction conditions should match the conditions present in the matrix or be specified for a particular application such as a simulated gastrointestinal digestion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4191-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Speciation of selenium compounds from high selenium broccoli is affected by the extracting solution.
pubmed:affiliation
Department of Chemistry, University of North Dakota, Grand Forks, North Dakota 58202, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't