Linked Life Data

12847272

Source:http://linkedlifedata.com/resource/pubmed/id/12847272

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-7-8
pubmed:abstractText
Hydrolysis of surfactant phospholipids by secreted phospholipases A(2) (sPLA(2)) contributes to surfactant dysfunction in acute respiratory distress syndrome. The present study demonstrates that sPLA(2)-IIA, sPLA(2)-V, and sPLA(2)-X efficiently hydrolyze surfactant phospholipids in vitro. In contrast, sPLA(2)-IIC, -IID, -IIE, and -IIF have no effect. Since purified surfactant protein A (SP-A) has been shown to inhibit sPLA(2)-IIA activity, we investigated the in vitro effect of SP-A on the other active sPLA(2) and the consequences of sPLA(2)-IIA inhibition by SP-A on surfactant phospholipid hydrolysis. SP-A inhibits sPLA(2)-X activity, but fails to interfere with that of sPLA(2)-V. Moreover, in vitro inhibition of sPLA(2)-IIA-induces surfactant phospholipid hydrolysis correlates with the concentration of SP-A in surfactant. Intratracheal administration of sPLA(2)-IIA to mice causes hydrolysis of surfactant phosphatidylglycerol. Interestingly, such hydrolysis is significantly higher for SP-A gene-targeted mice, showing the in vivo inhibitory effect of SP-A on sPLA(2)-IIA activity. Administration of sPLA(2)-IIA also induces respiratory distress, which is more pronounced in SP-A gene-targeted mice than in wild-type mice. We conclude that SP-A inhibits sPLA(2) activity, which may play a protective role by maintaining surfactant integrity during lung injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
171
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
995-1000
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12847272-Airway Resistance, pubmed-meshheading:12847272-Animals, pubmed-meshheading:12847272-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:12847272-Group II Phospholipases A2, pubmed-meshheading:12847272-Hydrolysis, pubmed-meshheading:12847272-Immunoblotting, pubmed-meshheading:12847272-Intubation, Intratracheal, pubmed-meshheading:12847272-Male, pubmed-meshheading:12847272-Mice, pubmed-meshheading:12847272-Mice, Inbred C57BL, pubmed-meshheading:12847272-Mice, Knockout, pubmed-meshheading:12847272-Phosphatidylcholines, pubmed-meshheading:12847272-Phosphatidylglycerols, pubmed-meshheading:12847272-Phospholipases A, pubmed-meshheading:12847272-Phospholipids, pubmed-meshheading:12847272-Pulmonary Surfactant-Associated Protein A, pubmed-meshheading:12847272-Respiratory Distress Syndrome, Adult
pubmed:year
2003
pubmed:articleTitle
Inhibitory effects of surfactant protein A on surfactant phospholipid hydrolysis by secreted phospholipases A2.
pubmed:affiliation
Unité de Défense Innée et Inflammation, Institut Pasteur, Institut National de la Santé et de la Recherche Médicale E 336, Paris, France.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't