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rdf:type |
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lifeskim:mentions |
umls-concept:C0023516,
umls-concept:C0039194,
umls-concept:C0242607,
umls-concept:C0946292,
umls-concept:C1334043,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1879547,
umls-concept:C2697616
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pubmed:issue |
2
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pubmed:dateCreated |
2003-7-8
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pubmed:abstractText |
Engagement of the T cell with Ag on an APC results in a series of immediate signaling events emanating from the stimulation of the TCR. These events include the induced phosphorylation of a number of cellular proteins with a subsequent increase in intracellular calcium and the restructuring of the microtubule and actin cytoskeleton within the T cell. This restructuring of the cytoskeleton culminates in the polarization of the T cell's secretory apparatus toward the engaging APC, allowing the T cell to direct secretion of cytokines toward the appropriate APC. This polarization can be monitored by analyzing the position of the microtubule-organizing center (MTOC), as it moves toward the interface of the T cell and APC. The requirements for MTOC polarization were examined at a single-cell level by studying the interaction of a Jurkat cell line expressing a fluorescently labeled MTOC with Staphylococcal enterotoxin superantigen-bound Raji B cell line, which served as the APC. We found that repolarization of the MTOC substantially followed fluxes in calcium. We also used immobilized anti-TCR mAb and Jurkat signaling mutants, defective in TCR-induced calcium increases, to determine whether signaling components that are necessary for a calcium response also play a role in MTOC polarization. We found that zeta-associated protein-70 as well as its substrate adaptor proteins linker for activation of T cells and Src homology 2 domain-containing leukocyte protein-76 are required for MTOC polarization. Moreover, our studies revealed that a calcium-dependent event not requiring calcineurin or calcium/calmodulin-dependent kinase is required for TCR-induced polarization of the MTOC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Benzylamines,
http://linkedlifedata.com/resource/pubmed/chemical/CD3E protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcineurin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/KN 93,
http://linkedlifedata.com/resource/pubmed/chemical/LAT protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/SLP-76 signal Transducing adaptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/Tacrolimus,
http://linkedlifedata.com/resource/pubmed/chemical/ZAP-70 Protein-Tyrosine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/ZAP70 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
860-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12847255-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:12847255-Antibodies, Monoclonal,
pubmed-meshheading:12847255-Antigen-Presenting Cells,
pubmed-meshheading:12847255-Antigens, CD3,
pubmed-meshheading:12847255-Benzylamines,
pubmed-meshheading:12847255-Calcineurin,
pubmed-meshheading:12847255-Calcium,
pubmed-meshheading:12847255-Calcium Signaling,
pubmed-meshheading:12847255-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:12847255-Carrier Proteins,
pubmed-meshheading:12847255-Cell Polarity,
pubmed-meshheading:12847255-Humans,
pubmed-meshheading:12847255-Jurkat Cells,
pubmed-meshheading:12847255-Lymphocyte Activation,
pubmed-meshheading:12847255-Membrane Proteins,
pubmed-meshheading:12847255-Microtubule-Organizing Center,
pubmed-meshheading:12847255-Phosphoproteins,
pubmed-meshheading:12847255-Protein-Tyrosine Kinases,
pubmed-meshheading:12847255-Receptors, Antigen, T-Cell,
pubmed-meshheading:12847255-Sulfonamides,
pubmed-meshheading:12847255-T-Lymphocytes,
pubmed-meshheading:12847255-Tacrolimus,
pubmed-meshheading:12847255-Tumor Cells, Cultured,
pubmed-meshheading:12847255-ZAP-70 Protein-Tyrosine Kinase
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pubmed:year |
2003
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pubmed:articleTitle |
Linker for activation of T cells, zeta-associated protein-70, and Src homology 2 domain-containing leukocyte protein-76 are required for TCR-induced microtubule-organizing center polarization.
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pubmed:affiliation |
Department of Medicine, Howard Hughes Medical Institute, Rosalind Russell Center for Medical Research in Arthritis, University of California, San Francisco, CA 94143, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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