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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-7-8
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pubmed:abstractText |
The establishment of clonally variable expression of MHC class I-specific receptors by NK cells is not well understood. The Ly-49A receptor is used by approximately 20% of NK cells, whereby most cells express either the maternal or paternal allele and few express simultaneously both alleles. We have previously shown that NK cells expressing Ly-49A were reduced or almost absent in mice harboring a single or no functional allele of the transcription factor T cell factor-1 (TCF-1), respectively. In this study, we show that enforced expression of TCF-1 in transgenic mice yields an expanded Ly-49A subset. Even though the frequencies of Ly-49A(+) NK cells varied as a function of the TCF-1 dosage, the relative abundance of mono- and biallelic Ly-49A cells was maintained. Mono- and biallelic Ly-49A NK cells were also observed in mice expressing exclusively a transgenic TCF-1, i.e., expressing a fixed amount of TCF-1 in all NK cells. These findings suggest that Ly-49A acquisition is a stochastic event due to limiting TCF-1 availability, rather than the consequence of clonally variable expression of the endogenous TCF-1 locus. Efficient Ly-49A acquisition depended on the expression of a TCF-1 isoform, which included a domain known to associate with the TCF-1 coactivator beta-catenin. Indeed, the proximal Ly-49A promoter was beta-catenin responsive in reporter gene assays. We thus propose that Ly-49A receptor expression is induced from a single allele in occasional NK cells due to a limitation in the amount of a transcription factor complex requiring TCF-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Catnb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphoid Enhancer-Binding Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, NK Cell Lectin-Like,
http://linkedlifedata.com/resource/pubmed/chemical/T Cell Transcription Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/TCF7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tcf7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
769-75
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12847244-Alleles,
pubmed-meshheading:12847244-Animals,
pubmed-meshheading:12847244-Antigens, Ly,
pubmed-meshheading:12847244-Cytoskeletal Proteins,
pubmed-meshheading:12847244-DNA-Binding Proteins,
pubmed-meshheading:12847244-Dose-Response Relationship, Immunologic,
pubmed-meshheading:12847244-Gene Expression Regulation,
pubmed-meshheading:12847244-Gene Rearrangement,
pubmed-meshheading:12847244-Humans,
pubmed-meshheading:12847244-Killer Cells, Natural,
pubmed-meshheading:12847244-Lectins, C-Type,
pubmed-meshheading:12847244-Lymphoid Enhancer-Binding Factor 1,
pubmed-meshheading:12847244-Mice,
pubmed-meshheading:12847244-Mice, Inbred BALB C,
pubmed-meshheading:12847244-Mice, Inbred C57BL,
pubmed-meshheading:12847244-Mice, Inbred DBA,
pubmed-meshheading:12847244-Mice, Transgenic,
pubmed-meshheading:12847244-Promoter Regions, Genetic,
pubmed-meshheading:12847244-Protein Isoforms,
pubmed-meshheading:12847244-Receptors, Immunologic,
pubmed-meshheading:12847244-Receptors, NK Cell Lectin-Like,
pubmed-meshheading:12847244-T Cell Transcription Factor 1,
pubmed-meshheading:12847244-Trans-Activators,
pubmed-meshheading:12847244-Transcription Factors,
pubmed-meshheading:12847244-Transfection,
pubmed-meshheading:12847244-beta Catenin
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pubmed:year |
2003
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pubmed:articleTitle |
Initiation and limitation of Ly-49A NK cell receptor acquisition by T cell factor-1.
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pubmed:affiliation |
Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Epalinges, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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