Linked Life Data

12842486

Source:http://linkedlifedata.com/resource/pubmed/id/12842486

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-7-4
pubmed:abstractText
Plasmodium falciparum drug resistance is a major problem in malaria endemic areas. Molecular markers and in vitro tests have been developed to study and monitor drug resistance. However, none, used alone, can provide sufficient data concerning the level of drug resistance and to issue precise guidelines for drug use policies in endemic areas. We propose real-time PCR for the simultaneous detection of pfcrt and pfmdr1 genes mutations and to determine the half-maximal inhibitory response (IC(50)) of antimalarial drug. Using hybridization probes and SybrGreen technology on LightCycler instrument, point mutations of pfcrt and pfmdr1 genes have been successfully detected in 161 human blood samples and determination of IC values was applied to chloroquine-sensitive and chloroquine-resistant strains. Moreover, mixed infections caused by P. falciparum clones with wild-type or mutant alleles could be efficiency separated. The aim of this study was not to provide definitive data concerning the rate of mutations in an endemic area, but to describe a powerful method allowing the quantification of DNA for IC(50) determination and the detection of major pfmdr1 and pfcrt mutations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0167-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
391-401
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12842486-ATP-Binding Cassette Transporters, pubmed-meshheading:12842486-Animals, pubmed-meshheading:12842486-Antimalarials, pubmed-meshheading:12842486-Chloroquine, pubmed-meshheading:12842486-DNA, Protozoan, pubmed-meshheading:12842486-Drug Resistance, pubmed-meshheading:12842486-Fluorescence Resonance Energy Transfer, pubmed-meshheading:12842486-Genetic Variation, pubmed-meshheading:12842486-Humans, pubmed-meshheading:12842486-Inhibitory Concentration 50, pubmed-meshheading:12842486-Malaria, Falciparum, pubmed-meshheading:12842486-Organic Chemicals, pubmed-meshheading:12842486-Plasmodium falciparum, pubmed-meshheading:12842486-Point Mutation, pubmed-meshheading:12842486-Polymerase Chain Reaction, pubmed-meshheading:12842486-Polymorphism, Genetic, pubmed-meshheading:12842486-Protozoan Proteins
pubmed:year
2003
pubmed:articleTitle
Real-time PCR for chloroquine sensitivity assay and for pfmdr1-pfcrt single nucleotide polymorphisms in Plasmodium falciparum.
pubmed:affiliation
Laboratoire de Parasitologie, Mycologie Med.et Pathologie Exotique, Hospices Civils de Lyon, Faculté de Médecine, Université Claude Bernard, E.A. 3087, Lyon, France. fdmonb@rockefeller.univ-lyonl.fr
pubmed:publicationType
Journal Article