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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-7-4
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pubmed:abstractText |
In the thymi of WT1-transgenic (Tg) mice with the 17AA+/KTS- spliced form of the Wilms tumor gene WT1 driven by the lck promoter, the frequencies of CD4-CD8- double-negative (DN) thymocytes were significantly increased relative to those in normal littermates. Of the 4 subsets of CD4-CD8- DN thymocytes, the DN1 (CD44+CD25-) subset increased in both frequency and absolute cell number, whereas the DN2 (CD44+CD25+) and DN3 (CD44-CD25+) subsets decreased, indicating the blocking of thymocyte differentiation from the DN1 to the DN2 subsets. Furthermore, CD4-CD8+ T-cell receptor (TCR) -gammadelta T-cells increased in both frequency and absolute cell number in the spleen and peripheral blood of the WT1-Tg mice relative to those of normal littermates. The CD8 molecules of these CD4-CD8+ TCRgammadelta T-cells were CD8alphabeta, suggesting that they originated from the thymus. These results are the first direct evidence demonstrating that the WT1 gene is involved in the development and differentiation of T-lineage cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0925-5710
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pubmed:author |
pubmed-author:AsadaMomotaroM,
pubmed-author:HosenNaokiN,
pubmed-author:KanatoKeisukeK,
pubmed-author:KawakamiManabuM,
pubmed-author:KawasakiKotomiK,
pubmed-author:KawaseIchiroI,
pubmed-author:KishimotoYukikoY,
pubmed-author:LiHanfenH,
pubmed-author:MasudaTomokiT,
pubmed-author:MelchersFritzF,
pubmed-author:MiyazakiToruT,
pubmed-author:MurakamiMasakiM,
pubmed-author:NakajimaHirokoH,
pubmed-author:NishidaSumiyukiS,
pubmed-author:OgawaHiroyasuH,
pubmed-author:OjiYusukeY,
pubmed-author:OkaYoshihiroY,
pubmed-author:SugiyamaHaruoH,
pubmed-author:TsuboiAkihiroA,
pubmed-author:YamagamiTamotsuT,
pubmed-author:YusaSei-ichiS
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
463-70
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12841384-Animals,
pubmed-meshheading:12841384-Antigens, CD,
pubmed-meshheading:12841384-Antigens, CD8,
pubmed-meshheading:12841384-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12841384-Cell Count,
pubmed-meshheading:12841384-Cell Differentiation,
pubmed-meshheading:12841384-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:12841384-Mice,
pubmed-meshheading:12841384-Mice, Transgenic,
pubmed-meshheading:12841384-Promoter Regions, Genetic,
pubmed-meshheading:12841384-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:12841384-T-Lymphocyte Subsets,
pubmed-meshheading:12841384-T-Lymphocytes,
pubmed-meshheading:12841384-Thymus Gland,
pubmed-meshheading:12841384-WT1 Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
The lck promoter-driven expression of the Wilms tumor gene WT1 blocks intrathymic differentiation of T-lineage cells.
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pubmed:affiliation |
Departments of Clinical Laboratory Science, Osaka University Medical School, Suita City, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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