Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-4-6
pubmed:databankReference
pubmed:abstractText
We describe the complete nucleotide sequence of a full length cDNA clone encoding a new mouse zinc finger protein gene, Zfp-38 and localize it on chromosome 5 by the interspecific backcross analysis. The N-terminal domain of the Zfp-38 protein (64 kDa) contains 358 amino acids and the C-terminal domain of 197 residues encodes 7 zinc fingers. We also present evidence that Zfp-38 is a strong transcriptional activator. The transactivation domain was localized in the non finger region and a fusion protein containing 112 amino acid residues from this region of the Zfp-38 and the DNA binding domain of the yeast Gal 4 protein, very efficiently transactivated the expression of a reporter CAT plasmid, harboring the Gal4 target site. By in situ hybridization and northern blotting technique, the Zfp-38 transcript can be detected at a highly elevated level during spermatogenesis. Its expression accompanies the progression from pachytene spermatocytes to round spermatids. The undifferentiated spermatogonia or the haploid elongated spermatid and the spermatozoa do not show any detectable level of the transcript. Interestingly, other tissues express low levels of a slightly shorter transcript with a different 5' end as determined by RNase protection. The presence of both a transcriptional activating domain and 7 DNA binding zinc fingers, coupled with the cell type(s) specific expression pattern, suggests that Zfp-38 has the potential to regulate transcription during spermatogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:geneSymbol
Zfp-38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-42
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1284028-Amino Acid Sequence, pubmed-meshheading:1284028-Animals, pubmed-meshheading:1284028-Base Sequence, pubmed-meshheading:1284028-Blotting, Northern, pubmed-meshheading:1284028-Chromosome Mapping, pubmed-meshheading:1284028-Crosses, Genetic, pubmed-meshheading:1284028-DNA-Binding Proteins, pubmed-meshheading:1284028-In Situ Hybridization, pubmed-meshheading:1284028-Male, pubmed-meshheading:1284028-Mice, pubmed-meshheading:1284028-Mice, Inbred C57BL, pubmed-meshheading:1284028-Molecular Sequence Data, pubmed-meshheading:1284028-Poly A, pubmed-meshheading:1284028-RNA, pubmed-meshheading:1284028-RNA, Messenger, pubmed-meshheading:1284028-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:1284028-Spermatogenesis, pubmed-meshheading:1284028-Testis, pubmed-meshheading:1284028-Trans-Activators, pubmed-meshheading:1284028-Transcription, Genetic, pubmed-meshheading:1284028-Transcriptional Activation, pubmed-meshheading:1284028-Transfection, pubmed-meshheading:1284028-Up-Regulation, pubmed-meshheading:1284028-Zinc Fingers
pubmed:year
1992
pubmed:articleTitle
The ubiquitous transactivator Zfp-38 is upregulated during spermatogenesis with differential transcription.
pubmed:affiliation
Max Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Göttingen, FRG.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't