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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2003-7-3
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pubmed:abstractText |
It has been postulated that reactive oxygen species (ROS) may act as second messengers leading to nuclear factor (NF)-kappaB activation. This hypothesis is mainly based on the findings that N-acetyl-L-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), compounds recognized as potential antioxidants, can inhibit NF-kappaB activation in a wide variety of cell types. Here we reveal that both NAC and PDTC inhibit NF-kappaB activation independently of antioxidative function. NAC selectively blocks tumor necrosis factor (TNF)-induced signaling by lowering the affinity of receptor to TNF. PDTC inhibits the IkappaB-ubiquitin ligase activity in the cell-free system where extracellular stimuli-regulated ROS production does not occur. Furthermore, we present evidence that endogenous ROS produced through Rac/NADPH oxidase do not mediate NF-kappaB signaling, but instead lower the magnitude of its activation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10022882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10072378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10097128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10225421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10228155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10346818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10385605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10652294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10699758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10801793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-10837071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-11006087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-11134170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-11402028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-11479295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-11983155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-12565873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-1314883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-14732063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-1643256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-1718003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-2065663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-2112750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-3414726,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-8439298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-8943367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-9034250,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12839997-9325328
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3356-66
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12839997-Acetylcysteine,
pubmed-meshheading:12839997-Base Sequence,
pubmed-meshheading:12839997-Cell Line,
pubmed-meshheading:12839997-DNA Primers,
pubmed-meshheading:12839997-Humans,
pubmed-meshheading:12839997-NF-kappa B,
pubmed-meshheading:12839997-Pyrrolidines,
pubmed-meshheading:12839997-Reactive Oxygen Species,
pubmed-meshheading:12839997-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:12839997-Signal Transduction,
pubmed-meshheading:12839997-Thiocarbamates,
pubmed-meshheading:12839997-Tumor Necrosis Factor-alpha
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pubmed:year |
2003
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pubmed:articleTitle |
Evidence that reactive oxygen species do not mediate NF-kappaB activation.
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pubmed:affiliation |
School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Tokyo 192-0392, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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