Source:http://linkedlifedata.com/resource/pubmed/id/12839973
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
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| pubmed:dateCreated |
2003-7-3
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| pubmed:abstractText |
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with poor prognosis. By representational difference analysis (RDA), a novel human gene designated SVH, up-regulated in the clinical HCC sample, was identified. The deduced SVH protein consisted of 343 amino acids with a transmembrane domain and an armadillo repeat. Northern blot revealed that SVH was expressed in most human adult tissues. Four variants of SVH, SVH-A, -B, -C, and -D, resulting from alternative splicing in the coding region of the SVH transcript, were observed and were all localized in endoplasmic reticulum (ER). Up-regulation of SVH-B, but not the other variants, was evident in about 60% (28 of 46) of HCC samples, detected by quantitative real-time PCR. Human liver cell line QSG-7701, transfected with SVH-B, acquired an accelerated growth rate and tumorigenicity in nude mice, whereas inhibition of SVH-B in hepatoma cell line BEL-7404, using antisense oligodeoxynucleotides, induced apoptosis. It is suggested that the splicing variants of SVH have distinct biological functions, and SVH-B may play an important role in hepatocarcinogenesis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
0008-5472
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
63
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3775-82
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12839973-Alternative Splicing,
pubmed-meshheading:12839973-Amino Acid Sequence,
pubmed-meshheading:12839973-Base Sequence,
pubmed-meshheading:12839973-Carcinoma, Hepatocellular,
pubmed-meshheading:12839973-Cell Division,
pubmed-meshheading:12839973-Cloning, Molecular,
pubmed-meshheading:12839973-DNA, Complementary,
pubmed-meshheading:12839973-DNA Primers,
pubmed-meshheading:12839973-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12839973-Genetic Variation,
pubmed-meshheading:12839973-Humans,
pubmed-meshheading:12839973-Liver Neoplasms,
pubmed-meshheading:12839973-Molecular Sequence Data,
pubmed-meshheading:12839973-Neoplasm Proteins,
pubmed-meshheading:12839973-Neoplasm Staging,
pubmed-meshheading:12839973-Plasmids,
pubmed-meshheading:12839973-Recombinant Proteins,
pubmed-meshheading:12839973-Sequence Alignment,
pubmed-meshheading:12839973-Sequence Homology, Amino Acid,
pubmed-meshheading:12839973-Trans-Activators,
pubmed-meshheading:12839973-Transfection
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| pubmed:year |
2003
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| pubmed:articleTitle |
A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas.
|
| pubmed:affiliation |
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 200031 Shanghai, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|