| pubmed-article:12838502 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C1565860 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C1705323 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C0206116 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C0034836 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C0300926 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:12838502 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:12838502 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12838502 | pubmed:dateCreated | 2003-7-2 | lld:pubmed |
| pubmed-article:12838502 | pubmed:abstractText | We have studied modulation of purinergic receptors (P(2Y) and P(2X) subtypes) on changes in intracellular Ca(2+) [Ca(2+)](i) and expression and production of COX-2 in human microglia. Measurements using Ca(2+)-sensitive spectrofluorometry showed adenosine triphosphate (ATP) to cause rapid transient increases in [Ca(2+)](i). Application of ATP plus the P(2X) antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), or treatment with adenosine diphosphate-beta-S (ADP-beta-S), a selective P(2Y) agonist, led to a considerable prolongation in [Ca(2+)](i) responses compared with ATP. The prolonged time courses were consistent with sustained activation of store-operated channels (SOC) since SKF96365, an inhibitor of SOC, blocked this component of the response. RT-PCR data showed that microglia expressed no COX-2 either constitutively or following treatment of cells with ATP (100 microM for 8 h). However, treatment using ATP plus PPADS or with ADP-beta-S led to marked expression of COX-2. The enhanced COX-2 with ATP plus PPADS treatment was absent in the presence of SKF96365 or using Ca(2+)-free solution. Immunocytochemistry, using a specific anti-COX-2 antibody, also revealed a pattern of purinergic modulation whereby lack of P(2X) activation enhanced the production of COX-2 protein. These results suggest that modulation of subtypes of purinergic receptors regulates COX-2 in human microglia with a link involving SOC-mediated influx of Ca(2+). | lld:pubmed |
| pubmed-article:12838502 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12838502 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12838502 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12838502 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12838502 | pubmed:issn | 0894-1491 | lld:pubmed |
| pubmed-article:12838502 | pubmed:author | pubmed-author:McLarnonJames... | lld:pubmed |
| pubmed-article:12838502 | pubmed:author | pubmed-author:RyuJae KJK | lld:pubmed |
| pubmed-article:12838502 | pubmed:author | pubmed-author:KimSeung USU | lld:pubmed |
| pubmed-article:12838502 | pubmed:author | pubmed-author:ChoiHyun BHB | lld:pubmed |
| pubmed-article:12838502 | pubmed:author | pubmed-author:HongSeok HSH | lld:pubmed |
| pubmed-article:12838502 | pubmed:copyrightInfo | Copyright 2003 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:12838502 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12838502 | pubmed:volume | 43 | lld:pubmed |
| pubmed-article:12838502 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12838502 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12838502 | pubmed:pagination | 95-103 | lld:pubmed |
| pubmed-article:12838502 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12838502 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12838502 | pubmed:articleTitle | Differential activation of subtype purinergic receptors modulates Ca(2+) mobilization and COX-2 in human microglia. | lld:pubmed |
| pubmed-article:12838502 | pubmed:affiliation | Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada. | lld:pubmed |
| pubmed-article:12838502 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12838502 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:12838502 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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