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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-3-16
|
| pubmed:abstractText |
Hepatitis C virus (HCV) replication was assessed before and during alpha-interferon (IFN) treatment in 22 anti-HCV positive patients with posttransfusion or sporadic chronic hepatitis (CH). Eleven patients were "responders" and 11 patients "non-responders" to IFN. Thirteen anti-HCV negative healthy subjects and five anti-HCV negative patients with autoimmune CH served as controls. Serum HCV-RNA was detected by the polymerase chain reaction (PCR) in all untreated anti-HCV positive patients but in none of the anti-HCV negative subjects. PCR primers from the 5'-noncoding (NC) region were more sensitive than primers from a non-structural (NS5) region in detecting HCV-RNA (21/22, 95% vs. 7/22, 32%, respectively). Positive strand HCV-RNA titre and positivity rate for the negative strand were similar in responders and non-responders before IFN treatment, as well as anti-c100-3 titre by enzyme-linked immunosorbent assay (ELISA), and anti-5-1-1, anti-c33c, anti-c22 positivity rate by immunoblot assay (RIBA). HCV-RNA positivity by both NC and NS primers was more frequent before IFN among responders. During IFN treatment, serum HCV-RNA was detectable, mostly at low titres, in 1 (NC positive) of the 11 responders and in 9 (4 NS positive and 5 NC positive) of the 11 non-responders. Among the four non-responders who were NS positive during IFN, three were NC positive before IFN. Serum HCV-RNA was always found in our post-transfusion or sporadic anti-HCV positive patients with CH. Viraemia generally decreased during IFN treatment, but no available HCV markers clearly distinguished responders from non-responders before IFN treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis C Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/interferon alfa-2b
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0146-6615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
200-6
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1283753-Adult,
pubmed-meshheading:1283753-Base Sequence,
pubmed-meshheading:1283753-Chronic Disease,
pubmed-meshheading:1283753-DNA, Single-Stranded,
pubmed-meshheading:1283753-Female,
pubmed-meshheading:1283753-Hepacivirus,
pubmed-meshheading:1283753-Hepatitis Antibodies,
pubmed-meshheading:1283753-Hepatitis C,
pubmed-meshheading:1283753-Hepatitis C Antibodies,
pubmed-meshheading:1283753-Humans,
pubmed-meshheading:1283753-Interferon-alpha,
pubmed-meshheading:1283753-Male,
pubmed-meshheading:1283753-Middle Aged,
pubmed-meshheading:1283753-Molecular Sequence Data,
pubmed-meshheading:1283753-Polymerase Chain Reaction,
pubmed-meshheading:1283753-RNA, Viral,
pubmed-meshheading:1283753-Recombinant Proteins,
pubmed-meshheading:1283753-Sensitivity and Specificity,
pubmed-meshheading:1283753-Viremia,
pubmed-meshheading:1283753-Virus Replication
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Serum hepatitis C virus (HCV)-RNA and response to alpha-interferon in anti-HCV positive chronic hepatitis.
|
| pubmed:affiliation |
Divisione di Medicina Interna, Ospedale V. Cervello, Palermo, Italy.
|
| pubmed:publicationType |
Journal Article
|