Linked Life Data

12832804

Source:http://linkedlifedata.com/resource/pubmed/id/12832804

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2003-6-30
pubmed:abstractText
Determination of the binding positions of light metal ions in the structure of oligonucleotides remains a challenge owing to their irregular coordination geometries, low electron densities and mobility in the crystals. To study the interactions between monovalent cations and DNA, the heavier alkali metal ion Rb(+) was used to replace Na(+) and K(+) in the Z-DNA crystal and the structure was analyzed using X-ray cryocrystallography. The resolution of the Rb-d(CGCGCG)(2) crystal data set is 1.76 A, with an R(merge) of 0.061. The final residual factor (R factor) for the crystal structure is 0.175. The positions of 16 Rb(+) ions were tentatively assigned, with most of them having partial occupancies. Three rubidium ions with full occupancies coordinated to two DNA double strands were located in the Z-DNA crystal. The results of this work suggest that there are numerous relatively low-energy sites for binding of monovalent cations to Z-DNA and that these sites are cation-water hybrid positions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1336-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
X-ray crystallographic study of the possible binding sites of the monovalent cations in the Z-DNA structure.
pubmed:affiliation
Department of Chemistry, Hunter College of The City University of New York, New York, NY 10071, USA. xcdong@nankai.edu.cn
pubmed:publicationType
Journal Article