Linked Life Data

12832800

Source:http://linkedlifedata.com/resource/pubmed/id/12832800

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2003-6-30
pubmed:abstractText
DFF45/ICAD, which forms a heterodimer with DFF40/CAD as its DNase inhibitor and chaperone, plays a key role in nuclei DNA fragmentation in apoptosis. Several fragments from the C-terminal region of DFF45/ICAD have been cloned and expressed in Escherichia coli as His-tagged proteins. After purification to homogeneity, the recombinant proteins of three fragments were crystallized by the hanging-drop vapor-diffusion method. Of these, a crystal of DFF45c1 diffracted to 3.4 A in a capillary at 277 K and crystals of DFF45c2 diffracted to 3.2 A at cryotemperature using synchrotron radiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1323-6
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Expression, purification, crystallization of fragments from the C-terminal region of DFF45/ICAD.
pubmed:affiliation
Life Science College, Peking University, Beijing 100871, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't