Linked Life Data

12832780

Source:http://linkedlifedata.com/resource/pubmed/id/12832780

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2003-6-30
pubmed:abstractText
Quinolinic acid phosphoribosyltransferase (NadC; EC 2.4.2.19) is the key enzyme of NAD(+) biosynthesis in both prokaryotes and eukaryotes. NadC catalyzes the decarboxylation of quinolinic acid (QA) to produce nicotinic acid mononucleotide (NAMN), an intermediate in NAD synthesis. NadCs of Helicobacter pylori appeared to be a hexamer during the purification procedure. Three different complexes of NadC, with QA, NAMN and phthalic acid (PA), an analogue of QA, were crystallized at 294 +/- 1 K using the hanging-drop vapour-diffusion method. The QA complex crystal was found to belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 148.8, c = 145.7 A, alpha = beta = gamma = 90 degrees. Diffraction data were collected from the NadC-substrate and NadC-substrate analogue complexes to resolutions of 2.3 A (QA), 2.8 A (PA) and 3.3 A (NAMN) using synchrotron X-ray radiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1265-6
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori.
pubmed:affiliation
Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't