Linked Life Data

12831534

Source:http://linkedlifedata.com/resource/pubmed/id/12831534

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-6-30
pubmed:abstractText
Bcl-X(L) is a pro-survival member of the Bcl-2 family that plays an important role in apoptosis regulation. As a first step to carry out the molecular characterization of this protein, we have generated five monoclonal antibodies (MAbs) directed against synthetic peptides corresponding to the flexible loop region of human and murine Bcl-X(L). These antibodies display species specificity and are suitable for Western blotting, immunofluorescence labeling, and immunoprecipitation. These results suggest that these antibodies can serve as important tools for the detection and purification of endogenous and ectopically expressed human and murine Bcl-X(L).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1536-8599
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Generation and characterization of species-specific anti-Bcl-X(L) monoclonal antibodies.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA. hsu@musc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.