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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-6-27
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pubmed:abstractText |
Wnt signaling mediated by beta-catenin plays crucial roles in the development of hepatocellular carcinoma and other cancers such as colorectal cancer. beta-Catenin associates with T-cell factor (TCF) transcription factors and functions as a transcriptional activator in the nucleus. By protein interaction screening, we identified EBP50, a cytoplasmic protein with 2 PDZ domains, as a beta-catenin-associating molecule. EBP50 interacted with beta-catenin through its carboxyl-PDZ domain in vitro and in vivo. Northern blot and RT-PCR analysis revealed an increase of EBP50 messenger RNA (mRNA) in hepatocellular carcinoma (HCC) cell lines and surgical specimens of human HCC. Over-expression of EBP50 protein with focal nuclear localization was detected in human HCC. In human HCC and colorectal cancer cell lines, EBP50 enhanced beta-catenin/TCF-dependent transcription in a dose-dependent manner. In an HCC cell line, over-expression of the carboxyl PDZ domain resulted in a decrease of endogenous beta-catenin/TCF transactivation. EBP50 promoted beta-catenin-mediated transactivation only in cells in which beta-catenin was already stabilized, suggesting that EBP50 may work with stabilized beta-catenin for transcriptional regulation. In conclusion, the EBP50/beta-catenin complex promotes Wnt signaling, and over-expression of EBP50 may work cooperatively with beta-catenin in the development of liver cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-hydrogen exchanger...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0270-9139
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
178-86
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12830000-Carcinoma, Hepatocellular,
pubmed-meshheading:12830000-Carrier Proteins,
pubmed-meshheading:12830000-Cloning, Molecular,
pubmed-meshheading:12830000-Cytoskeletal Proteins,
pubmed-meshheading:12830000-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12830000-HeLa Cells,
pubmed-meshheading:12830000-Humans,
pubmed-meshheading:12830000-Liver Neoplasms,
pubmed-meshheading:12830000-Phosphoproteins,
pubmed-meshheading:12830000-Protein Structure, Tertiary,
pubmed-meshheading:12830000-Proto-Oncogene Proteins,
pubmed-meshheading:12830000-Signal Transduction,
pubmed-meshheading:12830000-Sodium-Hydrogen Antiporter,
pubmed-meshheading:12830000-Trans-Activators,
pubmed-meshheading:12830000-Transcriptional Activation,
pubmed-meshheading:12830000-Wnt Proteins,
pubmed-meshheading:12830000-Zebrafish Proteins,
pubmed-meshheading:12830000-beta Catenin
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pubmed:year |
2003
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pubmed:articleTitle |
EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma.
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pubmed:affiliation |
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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