Linked Life Data

12825852

Source:http://linkedlifedata.com/resource/pubmed/id/12825852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-6-26
pubmed:abstractText
Members of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors are involved in the regulation of proliferation and differentiation of the mammary gland. In order to investigate the role of C/EBPalpha, -beta and -delta in breast cancer, we performed western blot analysis and partly immunohistochemistry in 75 mammary carcinomas, 10 normal mammary tissue samples and four mammary cell lines. Expression levels of both C/EBPalpha isoforms, C/EBPbeta isoforms LAP1, LAP2 (liver-enriched transcriptional activating proteins), and LIP (liver-enriched transcriptional inhibitory protein), and C/EBPdelta in the tumors were correlated with clinicopathological tumor parameters, expression of estrogen and progesterone receptors (ER, PR), Ki67 immunostaining, and expression of seven cell-cycle regulatory proteins which had been analyzed before. High C/EBPalpha and -delta protein levels correlated significantly with expression of cell-cycle promoters (cyclin D1 and E) and cell-cycle inhibitory proteins (Rb, p27, p16), but with none of the established prognostic parameters. In contrast, statistically significant relationships of the full-length C/EBPbeta isoform LAP1 and a negative estrogen receptor status, high grading, nodal involvement, and high cyclin E and p16 expression were found. For the shorter isoform LIP, correlations with an ER-negative phenotype and high Ki67 immunostaining were detected, and high histological grading (G3) correlated with lower LAP/LIP ratio. These results suggest that high C/EBPbeta expression might be involved in tumor progression and indicative of an unfavorable prognosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0167-6806
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-85
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12825852-Adult, pubmed-meshheading:12825852-Aged, pubmed-meshheading:12825852-Aged, 80 and over, pubmed-meshheading:12825852-Breast, pubmed-meshheading:12825852-Breast Neoplasms, pubmed-meshheading:12825852-Breast Neoplasms, Male, pubmed-meshheading:12825852-CCAAT-Enhancer-Binding Protein-alpha, pubmed-meshheading:12825852-CCAAT-Enhancer-Binding Protein-beta, pubmed-meshheading:12825852-CCAAT-Enhancer-Binding Protein-delta, pubmed-meshheading:12825852-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:12825852-Carcinoma, pubmed-meshheading:12825852-Cell Cycle, pubmed-meshheading:12825852-Cell Differentiation, pubmed-meshheading:12825852-Chi-Square Distribution, pubmed-meshheading:12825852-Female, pubmed-meshheading:12825852-Gene Expression Regulation, pubmed-meshheading:12825852-Humans, pubmed-meshheading:12825852-Male, pubmed-meshheading:12825852-Middle Aged, pubmed-meshheading:12825852-Protein Isoforms, pubmed-meshheading:12825852-Statistics as Topic, pubmed-meshheading:12825852-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
Expression of the CCAAT/enhancer-binding proteins C/EBPalpha, C/EBPbeta and C/EBPdelta in breast cancer: correlations with clinicopathologic parameters and cell-cycle regulatory proteins.
pubmed:affiliation
Institute of Pathology, Department of Gynecopathology, University-Hospital Hamburg-Eppendorf (UKE), Hamburg, Germany. milde@uke.uni-hamburg.de
pubmed:publicationType
Journal Article, Comparative Study, In Vitro