Linked Life Data

12825350

Source:http://linkedlifedata.com/resource/pubmed/id/12825350

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-6-26
pubmed:abstractText
A complete sequence of the 10.3 kb silkworm rDNA repeat in Attacus ricini (also called as Samia cynthia ricini) was first determined here, and sequences coding for 18S, 5.8S and 28S were also mapped to rDNA unit. The precise transcription initiation site was determined by primer extension experiment. Intergenic spacer (IGS) in the length of 2670 bp in which transcription of 45s rRNA precursor starts was found to contain three distinct AT-rich (A + T > 75%) regions. The AT-richest region (from -320 to -3, A + T > 83%) is just in front of the transcription initiation site. Many short sequence homologies were also found to exist in the transcription initiation region. It clearly demonstrates that the nucleotide sequence of initiation region of rRNA shows great diversity among different species, but the general functional architectures show some conservation. Analyzing the IGS region of A. ricini may provide new insights into the mechanisms of the expression regulation of RNA polymerase I contributed genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1042-5179
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-101
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Complete sequence of the 10.3 kb silkworm Attacus ricini rDNA repeat, determination of the transcriptional initiation site and functional analysis of the intergenic spacer.
pubmed:affiliation
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biology Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't