Source:http://linkedlifedata.com/resource/pubmed/id/12824921
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-6-25
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| pubmed:abstractText |
The expression levels of the Wilms' tumor gene WT1 were examined in 36 cases of various types of human bone and soft-tissue sarcomas using quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). They included 12 malignant fibrous histiocytomas (MFH), 3 malignant peripheral nerve sheath tumors (MPNST), 6 synovial sarcomas (SyS), 4 myxoid liposarcomas (MyLS), one angiosarcoma (AGS), one clear cell sarcoma (CCS), and 9 osteosarcomas (OS). Eleven (92%) of 12 MFH, 2 (67%) of 3 MPNST, all (100%) of 6 SyS, 2 (50%) of 4 MyLS, one AGS, one CCS, and 5 (56%) of 9 OS cases overexpressed WT1 in the range of 1.4 x 10(-3)-3.9 x 10(-1) levels (WT1 expression level in K562 leukemic cells was defined as 1.0). Thus, 28 (78%) out of 36 various types of human bone and soft-tissue sarcomas overexpressed the WT1 gene. Immunohistochemical analysis showed positive staining for WT1 protein in all of 4 cases (one case each of MFH, MyLS, AGS and OS) with WT1 gene overexpression detected by RT-PCR analysis, demonstrating clearly that WT1 was expressed at the protein level in various types of human bone and soft-tissue sarcomas. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of 10 exons of the WT1 gene in 8 different sarcoma samples (3 MFH, one SyS, one MyLS, one AGS, and 2 OS). The present study demonstrates that various types of human bone and soft-tissue sarcomas frequently overexpress the wild-type WT1 gene, suggesting an important role of the wild-type WT1 gene in tumorigenesis of various types of human bone and soft-tissue sarcomas.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1347-9032
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| pubmed:author |
pubmed-author:AbenoSakieS,
pubmed-author:AozasaKatsuyukiK,
pubmed-author:AsadaMomotaroM,
pubmed-author:HosenNaokiN,
pubmed-author:IkebaAiA,
pubmed-author:KogaSatokoS,
pubmed-author:MyouiAkiraA,
pubmed-author:NakaNorifumiN,
pubmed-author:NakanoYokoY,
pubmed-author:NakatsukaShin-ichiS,
pubmed-author:OjiYusukeY,
pubmed-author:SugiyamaHaruoH,
pubmed-author:TakahashiEigoE,
pubmed-author:TamaiNoriyukiN,
pubmed-author:TomitaYasuhikoY,
pubmed-author:UedaTakafumiT,
pubmed-author:YoshikawaHidekiH
|
| pubmed:issnType |
Print
|
| pubmed:volume |
94
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
271-6
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12824921-Adult,
pubmed-meshheading:12824921-Aged,
pubmed-meshheading:12824921-Base Sequence,
pubmed-meshheading:12824921-Bone Neoplasms,
pubmed-meshheading:12824921-DNA Primers,
pubmed-meshheading:12824921-Female,
pubmed-meshheading:12824921-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12824921-Genes, Wilms Tumor,
pubmed-meshheading:12824921-Humans,
pubmed-meshheading:12824921-Immunohistochemistry,
pubmed-meshheading:12824921-Kidney Neoplasms,
pubmed-meshheading:12824921-Male,
pubmed-meshheading:12824921-Middle Aged,
pubmed-meshheading:12824921-RNA, Neoplasm,
pubmed-meshheading:12824921-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12824921-Sarcoma,
pubmed-meshheading:12824921-WT1 Proteins,
pubmed-meshheading:12824921-Wilms Tumor
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Overexpression of the Wilms' tumor gene WT1 in human bone and soft-tissue sarcomas.
|
| pubmed:affiliation |
Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Suita, Osaka.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|