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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-6-25
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pubmed:abstractText |
Genes encoding 2C T cell receptor (TCR) alpha, beta chains from H-2(b)-restricted L(d)-specific CD8(+) cells were successfully transduced into polyclonally activated CD8(+) cells by retroviral modification to generate antigen-specific cytotoxic T lymphocytes (CTL). Antigen-nonspecific CD8(+) T cells polyclonally expanded in the presence of interleukin (IL)-2, Th1 cytokines (interferon (IFN)-gamma and IL-12) and anti-IL-4 monoclonal antibody showed neither cytokine production nor cytotoxicity in response to L(d)-expressing P815 tumor cells. However, 2C-TCR gene-modified CD8(+) T cells exhibited both IFN-gamma production and cytotoxicity in response to P815 tumor cells. The antitumor activity of TCR gene-modified Tc1 cells was also demonstrated in vivo by Winn's assay. Thus, we have developed an efficient method to induce TCR gene-modified antigen-specific Tc1 cells that exhibit antitumor activity both in vitro and in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1347-9032
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pubmed:author |
pubmed-author:AbeHiroyukiH,
pubmed-author:ChamotoKenjiK,
pubmed-author:FujioKeishiK,
pubmed-author:FunamotoHiromiH,
pubmed-author:KitamuraToshioT,
pubmed-author:KodaToshiakiT,
pubmed-author:KosakaAkemiA,
pubmed-author:MatsuzakiJunkoJ,
pubmed-author:NishimuraTakashiT,
pubmed-author:TogashiYujiY,
pubmed-author:TsujiTakemasaT,
pubmed-author:YamamotoKazuhikoK
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
389-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12824910-Animals,
pubmed-meshheading:12824910-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12824910-Cytokines,
pubmed-meshheading:12824910-DNA-Binding Proteins,
pubmed-meshheading:12824910-Gene Therapy,
pubmed-meshheading:12824910-Lymphoma,
pubmed-meshheading:12824910-Mastocytoma,
pubmed-meshheading:12824910-Mice,
pubmed-meshheading:12824910-Mice, Inbred C57BL,
pubmed-meshheading:12824910-Mice, Knockout,
pubmed-meshheading:12824910-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:12824910-Retroviridae,
pubmed-meshheading:12824910-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:12824910-Tissue Therapy,
pubmed-meshheading:12824910-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
An efficient method to prepare T cell receptor gene-transduced cytotoxic T lymphocytes type 1 applicable to tumor gene cell-therapy.
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pubmed:affiliation |
Division of Immunoregulation, Section of Disease Control, Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo 060-0815, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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