|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2003-6-24
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
RNA editing produces mature trypanosome mitochondrial mRNAs by uridylate (U) insertion and deletion. In insertion editing, Us are added to the pre-mRNA by a 3' terminal uridylyl transferase (TUTase) activity. We report the identification of a TUTase activity that copurifies with in vitro editing and is catalyzed by the integral editosome protein TbMP57. TbMP57 catalyzes the addition of primarily a single U to single-stranded (ss) RNA and adds the number of Us specified by a guide RNA to insertion editing-like substrates. TbMP57 is distinct from a previously identified TUTase that adds many Us to ssRNA and which we find is neither a stable editosome component nor does it add Us to editing-like substrates. Recombinant TbMP57 specifically interacts with the editosome protein TbMP81, and this interaction enhances the TUTase activity. These results suggest that TbMP57 catalyzes U addition to pre-mRNA during editing.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Guide,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/TbMP81 protein, Trypanosoma brucei,
http://linkedlifedata.com/resource/pubmed/chemical/UDPglucose-Hexose-1-Phosphate...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1097-2765
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1525-36
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:12820966-Animals,
pubmed-meshheading:12820966-Catalysis,
pubmed-meshheading:12820966-Chromatography, Agarose,
pubmed-meshheading:12820966-Chromatography, Gel,
pubmed-meshheading:12820966-Chromatography, Ion Exchange,
pubmed-meshheading:12820966-Mitochondria,
pubmed-meshheading:12820966-Molecular Sequence Data,
pubmed-meshheading:12820966-Molecular Weight,
pubmed-meshheading:12820966-Protein Structure, Tertiary,
pubmed-meshheading:12820966-Protozoan Proteins,
pubmed-meshheading:12820966-RNA,
pubmed-meshheading:12820966-RNA, Guide,
pubmed-meshheading:12820966-RNA, Messenger,
pubmed-meshheading:12820966-RNA Editing,
pubmed-meshheading:12820966-RNA Precursors,
pubmed-meshheading:12820966-Recombinant Proteins,
pubmed-meshheading:12820966-Ribonucleoproteins,
pubmed-meshheading:12820966-Substrate Specificity,
pubmed-meshheading:12820966-Trypanosoma brucei brucei,
pubmed-meshheading:12820966-UDPglucose-Hexose-1-Phosphate Uridylyltransferase
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
TbMP57 is a 3' terminal uridylyl transferase (TUTase) of the Trypanosoma brucei editosome.
|
|
pubmed:affiliation |
Seattle Biomedical Research Institute, Seattle, WA 98109, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
