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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-6-24
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pubmed:abstractText |
We studied a well-selected population of patients with active rheumatoid arthritis (RA) or systemic lupus erythematosus (SLE) without immunosuppressive therapy. Control and patient peripheral blood mononuclear cells (PBMC) were incubated with IL-1beta, IL-10, TGF-beta or LPS for 20 h and the in vitro basal and stimulated secretions of IL-6, TNF-alpha, IL-1beta and IL-1ra were measured by ELISA. We found that in the SLE patients the basal secretion of IL-6 was significantly lower and that of IL-1ra significantly higher than in control subjects, while in the RA group the basal IL-1ra secretion was higher than in healthy subjects. SLE and RA PBMC responded to LPS and IL-1beta reaching higher cytokine secretion values than controls. The in vitro response of SLE and RA PBMC to TGFbeta was normal, while that to IL-10 was defective: IL-10 was able to stimulate the production of IL-6 and IL-1ra in PBMC from normal subjects, but it was unable to enhance IL-6 secretion in RA cells and it was also completely ineffective in inducing IL-1ra secretion in both SLE and RA PBMC. Our work add new data useful for the evaluation of IL-10 and IL-1ra as therapeutic agents in rheumatic diseases.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/IL1RN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0891-6934
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12820688-Adolescent,
pubmed-meshheading:12820688-Adult,
pubmed-meshheading:12820688-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:12820688-Arthritis, Rheumatoid,
pubmed-meshheading:12820688-Case-Control Studies,
pubmed-meshheading:12820688-Cytokines,
pubmed-meshheading:12820688-Female,
pubmed-meshheading:12820688-Humans,
pubmed-meshheading:12820688-Inflammation Mediators,
pubmed-meshheading:12820688-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:12820688-Interleukin-1,
pubmed-meshheading:12820688-Interleukin-10,
pubmed-meshheading:12820688-Interleukin-6,
pubmed-meshheading:12820688-Leukocytes, Mononuclear,
pubmed-meshheading:12820688-Lipopolysaccharides,
pubmed-meshheading:12820688-Lupus Erythematosus, Systemic,
pubmed-meshheading:12820688-Middle Aged,
pubmed-meshheading:12820688-Recombinant Proteins,
pubmed-meshheading:12820688-Sialoglycoproteins,
pubmed-meshheading:12820688-Transforming Growth Factor beta,
pubmed-meshheading:12820688-Tumor Necrosis Factor-alpha
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pubmed:year |
2003
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pubmed:articleTitle |
Effect of pro-inflammatory/anti-inflammatory agents on cytokine secretion by peripheral blood mononuclear cells in rheumatoid arthritis and systemic lupus erythematosus.
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pubmed:affiliation |
Institute of General Pathology, Catholic University, Largo Franesco Vito, 1, 00168 Rome, Italy.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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