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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2003-6-23
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pubmed:abstractText |
Shiga toxin type 2 (Stx2) produced by Escherichia coli O:157H7 can cause hemolytic-uremic syndrome in children, a disease for which there is neither a vaccine nor an effective treatment. This toxin consists of an enzymatically active A subunit and a pentameric B subunit responsible for the toxin binding to host cells, and also found to be immunogenic in rabbits. In this study we developed eukaryotic plasmids expressing the B subunit gene of Stx2 (pStx2B) and the B subunit plus the gene coding for the A subunit with an active-site deletion (pStx2 Delta A). Transfection of eukaryotic cells with these plasmids produced proteins of the expected molecular weight which reacted with specific monoclonal antibodies. Newborn and adult BALB/c mice immunized with two intramuscular injections of each plasmid, either alone or together with the same vector expressing the granulocyte and monocyte colony-stimulating factor (pGM-CSF), elicited a specific Th1-biased humoral response. The effect of pGM-CSF as an adjuvant plasmid was particularly notable in newborn mice and in pStx2B-vaccinated adult mice. Stx2-neutralizing activity, evaluated in vitro on VERO cell monolayers, correlated with in vivo protection. This is the first report using plasmids to induce a neutralizing humoral immune response against the Stx2.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10547430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10580044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10669329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10820272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10866130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10869769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10895014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-10946318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-11110647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-11133375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-11225323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-11228378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-11517435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-11803949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-12021709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-1730480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-1757536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-2047652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-2475438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-2644022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-3095828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-7806370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-8265577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-8562852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-8837611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-8879229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-8970972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9175848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9199412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9234543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9256266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9298210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9435018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819084-9652427
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3971-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12819084-Animals,
pubmed-meshheading:12819084-Antibodies, Bacterial,
pubmed-meshheading:12819084-Disease Models, Animal,
pubmed-meshheading:12819084-Escherichia coli Vaccines,
pubmed-meshheading:12819084-Hemolytic-Uremic Syndrome,
pubmed-meshheading:12819084-Immunoglobulin G,
pubmed-meshheading:12819084-Mice,
pubmed-meshheading:12819084-Mice, Inbred BALB C,
pubmed-meshheading:12819084-Neutralization Tests,
pubmed-meshheading:12819084-Plasmids,
pubmed-meshheading:12819084-Shiga Toxin 2,
pubmed-meshheading:12819084-Transfection,
pubmed-meshheading:12819084-Vaccines, DNA
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pubmed:year |
2003
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pubmed:articleTitle |
Development of DNA vaccines against hemolytic-uremic syndrome in a murine model.
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pubmed:affiliation |
Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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