Linked Life Data

12819018

Source:http://linkedlifedata.com/resource/pubmed/id/12819018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-23
pubmed:abstractText
A group of genes are highly expressed in normal germinal center (GC) B cells and GC B-cell-derived malignancies based on cDNA microarray analysis. Two new genes, GCET1 (germinal center B-cell expressed transcript 1) and GCET2, were cloned from selected expressed sequence tags (IMAGE clone 1334260 and 814622, respectively). GCET1 is located on chromosome 14q32 and has four splicing isoforms, of which the longest one is 1787 bp and encodes a 435-amino acid protein. GCET2 is located on 3q13.13, and the cloned fragment is 3270 bp, which encodes a protein of 178 amino acids. Blast search showed that GCET1 has a highly conserved serine proteinase inhibitor (SERPIN) domain and is located on a chromosomal locus containing seven other SERPIN family members. GCET2 is a likely homologue of the mouse gene M17, a GC-expressed transcript. Analysis of the GCET2 protein sequence indicated that it may be involved in signal transduction in the cytoplasm. Northern blot and real-time polymerase chain reaction analyses confirmed that GCET1 is highly restricted to normal GC B cells and GCB-cell-derived cell lines. Although GCET2 is also a useful marker for normal and neoplastic GC B cells, it has a wider range of expression including immature B and T cells. Real-time polymerase chain reaction assay showed that both GCET1 and GCET2 are preferentially expressed in follicular lymphoma and diffuse large B-cell lymphoma with GC B-cell differentiation, confirming previous microarray gene expression analysis, but neither one is entirely specific. Multiple markers are necessary to differentiate the GCB from the activated B-cell type of diffuse large B-cell lymphoma with a high degree of accuracy.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-10037822, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-10676951, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11069088, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11268149, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11283593, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11313912, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11722574, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11748286, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11757704, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11869899, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-11895757, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-12075054, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-12509382, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-159361, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-214220, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-4097745, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-68013, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7216541, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7531702, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7559394, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7573945, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7594458, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7608543, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-7981148, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-8152260, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-8600537, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-8624801, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-9031148, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-9204764, http://linkedlifedata.com/resource/pubmed/commentcorrection/12819018-9237932
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-44
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:12819018-Amino Acid Sequence, pubmed-meshheading:12819018-Animals, pubmed-meshheading:12819018-B-Lymphocytes, pubmed-meshheading:12819018-Base Sequence, pubmed-meshheading:12819018-Chromosomes, Human, Pair 14, pubmed-meshheading:12819018-Chromosomes, Human, Pair 3, pubmed-meshheading:12819018-Cloning, Molecular, pubmed-meshheading:12819018-Expressed Sequence Tags, pubmed-meshheading:12819018-Gene Expression Profiling, pubmed-meshheading:12819018-Gene Expression Regulation, pubmed-meshheading:12819018-Germinal Center, pubmed-meshheading:12819018-Humans, pubmed-meshheading:12819018-Lymphoma, Large B-Cell, Diffuse, pubmed-meshheading:12819018-Mice, pubmed-meshheading:12819018-Molecular Sequence Data, pubmed-meshheading:12819018-Neoplasm Proteins, pubmed-meshheading:12819018-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:12819018-Oncogene Proteins, pubmed-meshheading:12819018-Serpins
pubmed:year
2003
pubmed:articleTitle
Two newly characterized germinal center B-cell-associated genes, GCET1 and GCET2, have differential expression in normal and neoplastic B cells.
pubmed:affiliation
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska and The Metabolism Branch, National Cancer Institute, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.