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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-1-28
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pubmed:databankReference |
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pubmed:abstractText |
The gene encoding an antigenic polypeptide of Anisakis simplex larvae was studied using recombinant DNA techniques. cDNA synthesized from poly(A)-rich mRNA from A. simplex larvae was ligated into phage vector lambda gtll DNA and packaged in vitro. The phages were propagated on Escherichia coli and a lambda gtll expression library was constructed. A cDNA clone encoding a 42 kDa antigenic polypeptide was selected by immunoscreening of the library and identified by the epitope selection method. A clone containing cDNA for a 42 kDa protein was isolated. The gene encoding this 42 kDa antigenic polypeptide was characterized by DNA and RNA blot analysis using the cDNA as a probe. The gene was transcribed to mRNA with approximately 1400 nucleotides and translated to 42 kDa polypeptide. The antigenic beta-galactosidase fusion protein synthesized by bacteria had no cross-reactivity with other parasite-infected sera.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-149X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-32
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1281853-Amino Acid Sequence,
pubmed-meshheading:1281853-Animals,
pubmed-meshheading:1281853-Anisakis,
pubmed-meshheading:1281853-Antigens, Helminth,
pubmed-meshheading:1281853-Base Sequence,
pubmed-meshheading:1281853-Blotting, Northern,
pubmed-meshheading:1281853-Blotting, Southern,
pubmed-meshheading:1281853-Blotting, Western,
pubmed-meshheading:1281853-Cloning, Molecular,
pubmed-meshheading:1281853-DNA,
pubmed-meshheading:1281853-Epitopes,
pubmed-meshheading:1281853-Gene Expression Regulation,
pubmed-meshheading:1281853-Gene Library,
pubmed-meshheading:1281853-Helminth Proteins,
pubmed-meshheading:1281853-Larva,
pubmed-meshheading:1281853-Molecular Sequence Data,
pubmed-meshheading:1281853-Nucleic Acid Hybridization,
pubmed-meshheading:1281853-Precipitin Tests,
pubmed-meshheading:1281853-Protein Biosynthesis,
pubmed-meshheading:1281853-RNA, Messenger
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pubmed:year |
1992
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pubmed:articleTitle |
Molecular cloning of the cDNA encoding a 42 kDa antigenic polypeptide of Anisakis simplex larvae.
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pubmed:affiliation |
Department of Parasitology, Shinshu University School of Medicine, Nagano Prefecture, Japan.
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pubmed:publicationType |
Journal Article
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