Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-1-27
pubmed:abstractText
In human peripheral interstitial fluid, esterification of cholesterol by lecithin cholesterol acyltransferase (LCAT) was found to occur at a rate of only 10% of that in plasma (5.6 +/- 1.8 compared with 55.6 +/- 7.8 nmol/ml per h). Measurement of cholesterol esterification in the presence of excess reconstituted apoA-I HDL (rA-I HDL) revealed an LCAT activity in interstitial fluid of 24% of that in plasma, indicating that the low rate of esterification could not be caused by limiting mass of LCAT enzyme. When plasma was diluted to the same concentration as in interstitial fluid, the percent cholesterol esterification rate was the same as undiluted plasma and significantly higher than that of interstitial fluid. These findings led us to postulate that poor activation of LCAT in interstitial fluid may result from a change in conformation in apoA-I. To test this hypothesis, a monoclonal antibody AI-11 that inhibits apoA-I activation of LCAT was used to measure apoA-I in interstitial fluid and plasma. Antibody AI-11 recognized interstitial fluid apoA-I poorly, whereas a polyclonal antibody recognized interstitial fluid apoA-I normally. Incubation of antibody AI-11 with high density lipoprotein or rA-I HDL inhibited apoA-I activation of LCAT. We conclude that the altered conformation of apoA-I in interstitial fluid may render it a poor activator of LCAT.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-1638752, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-1721059, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-175978, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-182724, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2105091, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2125044, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2403299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2458351, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2579072, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2686603, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-2768238, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-3126809, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-3141545, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-3553402, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-3611967, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4019511, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4020293, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4114358, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4354500, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4368869, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4785134, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4821169, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4868034, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-4868699, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-5100059, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-6427214, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-6574494, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-6651916, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-6655363, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-6774331, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-6774335, http://linkedlifedata.com/resource/pubmed/commentcorrection/1281832-7400692
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2370-5
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Altered epitope expression of human interstitial fluid apolipoprotein A-I reduces its ability to activate lecithin cholesterol acyl transferase.
pubmed:affiliation
Louisiana State University Medical Center, Department of Physiology, New Orleans 70112-2822.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.