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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5089
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pubmed:dateCreated |
1993-1-21
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pubmed:abstractText |
Interferon-gamma (IFN-gamma) induces the transcription of the gene encoding a guanylate binding protein by activating a latent cytoplasmic factor, GAF (gamma-activated factor). GAF is translocated to the nucleus and binds a DNA element, the gamma-activated site. Through cross-linking and the use of specific antibodies GAF was found to be a 91-kilodalton DNA binding protein that was previously identified as one of four proteins in interferon-stimulated gene factor-3 (ISGF-3), a transcription complex activated by IFN-alpha. The IFN-gamma-dependent activation of the 91-kilodalton DNA binding protein required cytoplasmic phosphorylation of the protein on tyrosine. The 113-kilodalton ISGF-3 protein that is phosphorylated in response to IFN-alpha was not phosphorylated nor translocated to the nucleus in response to IFN-gamma. Thus the two different ligands result in tyrosine phosphorylation of different combinations of latent cytoplasmic transcription factors that then act at different DNA binding sites.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1808-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1281555-Animals,
pubmed-meshheading:1281555-Base Sequence,
pubmed-meshheading:1281555-Binding Sites,
pubmed-meshheading:1281555-Cell Line,
pubmed-meshheading:1281555-Cell Nucleus,
pubmed-meshheading:1281555-DNA-Binding Proteins,
pubmed-meshheading:1281555-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:1281555-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1281555-GTP-Binding Proteins,
pubmed-meshheading:1281555-Gene Expression Regulation,
pubmed-meshheading:1281555-Interferon-alpha,
pubmed-meshheading:1281555-Interferon-gamma,
pubmed-meshheading:1281555-Models, Biological,
pubmed-meshheading:1281555-Molecular Sequence Data,
pubmed-meshheading:1281555-Molecular Weight,
pubmed-meshheading:1281555-Oligodeoxyribonucleotides,
pubmed-meshheading:1281555-Phosphorylation,
pubmed-meshheading:1281555-Phosphotyrosine,
pubmed-meshheading:1281555-Promoter Regions, Genetic,
pubmed-meshheading:1281555-STAT1 Transcription Factor,
pubmed-meshheading:1281555-Signal Transduction,
pubmed-meshheading:1281555-Trans-Activators,
pubmed-meshheading:1281555-Transcription, Genetic,
pubmed-meshheading:1281555-Tyrosine
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pubmed:year |
1992
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pubmed:articleTitle |
Activation of transcription by IFN-gamma: tyrosine phosphorylation of a 91-kD DNA binding protein.
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pubmed:affiliation |
Rockefeller University, New York, NY 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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