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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1993-1-19
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pubmed:databankReference |
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pubmed:abstractText |
Using an expression cloning strategy, we isolated a cDNA encoding a human protein-tyrosine-phosphatase. Bacteria expressing the kinase domain of the keratinocyte growth factor receptor (bek/fibroblast growth factor receptor 2) were infected with a fibroblast cDNA library in a phagemid prokaryotic expression vector and screened with a monoclonal anti-phosphotyrosine antibody. Among several clones showing decreased anti-phosphotyrosine recognition, one displayed phosphatase activity toward the kinase in vitro. The 4.1-kilobase cDNA encoded a deduced protein of 185 amino acids with limited sequence similarity to the vaccinia virus phosphatase VH1. The purified recombinant protein dephosphorylated several activated growth factor receptors, as well as serine-phosphorylated casein, in vitro. Both serine and tyrosine phosphatase activities were completely abolished by mutagenesis of a single cysteine residue conserved in VH1 and the VH1-related (VHR) human protein. These properties suggest that VHR is capable of regulating intracellular events mediated by both tyrosine and serine phosphorylation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1310893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1318193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1321146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1412695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1519755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1650499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1655274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1657401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1657402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1659742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1695146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1741163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1825803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1828290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1836978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1846048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1848923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1852137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-1943760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2052597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2139203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2158859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2165484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2170109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2468999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2473838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2531695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2546149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2550140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2682257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-2834387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-3291115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-3313277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-3469660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-3841511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281549-5432063
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:geneSymbol |
VHR,
bek
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12170-4
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1281549-Amino Acid Sequence,
pubmed-meshheading:1281549-Base Sequence,
pubmed-meshheading:1281549-Cloning, Molecular,
pubmed-meshheading:1281549-Gene Expression,
pubmed-meshheading:1281549-Humans,
pubmed-meshheading:1281549-Molecular Sequence Data,
pubmed-meshheading:1281549-Phosphoprotein Phosphatases,
pubmed-meshheading:1281549-Phosphoserine,
pubmed-meshheading:1281549-Phosphotyrosine,
pubmed-meshheading:1281549-RNA, Messenger,
pubmed-meshheading:1281549-Restriction Mapping,
pubmed-meshheading:1281549-Sequence Alignment,
pubmed-meshheading:1281549-Substrate Specificity,
pubmed-meshheading:1281549-Tyrosine
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pubmed:year |
1992
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pubmed:articleTitle |
Expression cloning of a human dual-specificity phosphatase.
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pubmed:affiliation |
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Comparative Study
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