Linked Life Data

12815289

Source:http://linkedlifedata.com/resource/pubmed/id/12815289

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2003-6-19
pubmed:abstractText
To assess the potential of cell array technology, a cell array slide with 50 spots was designed specifically for automated analysis of changes in expression of cyclins A and B1 during the cell cycle at the cellular level. Cells harvested every 1 hour from 0 through 23 hours after synchronization by mitotic selection were spotted in duplicate on a glass slide. Each slide contained 48 spots representing 24 different cell cycle phases, and the remaining two spots were peripheral blood lymphocytes, which were included as negative control. This cell array provided temporal and spatial information related to changes in cyclin expression during the cell cycle in a single experiment. The present study indicates that expression analysis by cell array is novel approach for cell cycle studies. Furthermore, sophisticated multiplexed cell array technology has great potential for analyses of expression of specific genes during diverse cellular events at the cellular level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1381-5741
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle. An application of cell array system.
pubmed:affiliation
Department of Pathology, Yamaguchi University School of Medicine, Ube 755-8505, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies