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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1993-1-15
|
| pubmed:abstractText |
We previously reported that murine bone marrow cells activated by interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) had potent nonspecific natural suppressor (NS) cell activity. In the present study, we demonstrated that these activated NS cells released a soluble factor (or factors) capable of nonspecifically inhibiting T cell mitogenic responses. Consistent with the properties of transforming growth factor-beta (TGF-beta), treatment of the NS supernates with heat failed to denature the factor, and in fact significantly increased its suppressive activity. The NS suppressor factor strongly inhibited proliferation of the TGF-beta-sensitive tumor cell line, A549. Cytokine activation of suppressive activity correlated with the production of a 10- to 13-kDa protein, consistent with the size of TGF-beta and rIL-3 induced a sevenfold increase in TGF-beta transcription. Finally, neutralizing anti-TGF-beta antibody inhibited the suppressive activity of the supernates, indicating that TGF-beta was responsible for most, if not all, of the suppression expressed by these bone marrow NS cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0741-5400
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
52
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
596-601
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1281488-Animals,
pubmed-meshheading:1281488-Bone Marrow,
pubmed-meshheading:1281488-Bone Marrow Cells,
pubmed-meshheading:1281488-Cell Division,
pubmed-meshheading:1281488-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1281488-Female,
pubmed-meshheading:1281488-Humans,
pubmed-meshheading:1281488-Interferon-gamma,
pubmed-meshheading:1281488-Interleukin-3,
pubmed-meshheading:1281488-Lung Neoplasms,
pubmed-meshheading:1281488-Mice,
pubmed-meshheading:1281488-Mice, Inbred C57BL,
pubmed-meshheading:1281488-Neutralization Tests,
pubmed-meshheading:1281488-RNA,
pubmed-meshheading:1281488-Recombinant Proteins,
pubmed-meshheading:1281488-T-Lymphocytes, Regulatory,
pubmed-meshheading:1281488-Transforming Growth Factor beta,
pubmed-meshheading:1281488-Tumor Cells, Cultured
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Transforming growth factor-beta is the major mediator of natural suppressor cells derived from normal bone marrow.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|