Linked Life Data

12812314

Source:http://linkedlifedata.com/resource/pubmed/id/12812314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-6-18
pubmed:abstractText
CD3 antigen, formerly thought to be specific for T lymphocytes, has been found on gastric parietal cells in animals and humans. The common anti-CD3 antibodies recognize the epsilon subunit, which has a role in signal transduction. The aim of this study was to immunostain stomach specimens from humans and different animal species for CD3 antigen to determine if CD3 antigen is conserved across species and if CD3 antigen expression is altered in humans by use of certain drugs or the presence of gastritis. Gastric biopsies from 50 humans and necropsy sections from 13 different animals were immunostained using commercial anti CD3 epsilon antibodies on an automated immunostainer. Sections of stomach from four mice lacking the gastric H+,K(+)-ATPase alpha-subunit and four control mice were similarly immunostained. CD3 epsilon antigen expression in cytoplasm and cell membranes of gastric parietal cells was graded subjectively based on the number of positive cells. CD3 epsilon antigen was found on gastric parietal cells in all but one species studied, with varying expression in membranes and cytoplasm. There was a trend toward a decreased frequency of CD3+ cells in biopsies from patients on drugs (n = 23) compared to those on no drugs (n = 27). This trend was most marked in patients on H2 receptor antagonists. There was no correlation between CD3 expression and inflammation or Helicobacter pylori (H. pylori) infection. There was loss of CD3 expression in parietal cells in mice lacking the alpha-subunit of H+,K(+)-ATPase. These findings support previous observations that CD3 antigen is present in gastric parietal cells, and suggest that it may function in signal transduction during acid secretion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0344-0338
pubmed:author
pubmed:issnType
Print
pubmed:volume
199
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12812314-Adolescent, pubmed-meshheading:12812314-Adult, pubmed-meshheading:12812314-Aged, pubmed-meshheading:12812314-Aged, 80 and over, pubmed-meshheading:12812314-Animals, pubmed-meshheading:12812314-Antiemetics, pubmed-meshheading:12812314-Antigens, CD3, pubmed-meshheading:12812314-Child, pubmed-meshheading:12812314-Enzyme Inhibitors, pubmed-meshheading:12812314-Female, pubmed-meshheading:12812314-Gastritis, pubmed-meshheading:12812314-H(+)-K(+)-Exchanging ATPase, pubmed-meshheading:12812314-Histamine H2 Antagonists, pubmed-meshheading:12812314-Humans, pubmed-meshheading:12812314-Immunohistochemistry, pubmed-meshheading:12812314-Male, pubmed-meshheading:12812314-Middle Aged, pubmed-meshheading:12812314-Parietal Cells, Gastric, pubmed-meshheading:12812314-Receptors, Antigen, T-Cell, pubmed-meshheading:12812314-Signal Transduction, pubmed-meshheading:12812314-Species Specificity
pubmed:year
2003
pubmed:articleTitle
Expression of CD3 epsilon subunit in gastric parietal cells: a possible role in signal transduction?
pubmed:affiliation
Department of Pathology, UMASS Memorial Healthcare and Medical School, Worcester, MA 016585, USA. banner@ummhc.org
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.