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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
35
|
| pubmed:dateCreated |
1993-1-12
|
| pubmed:abstractText |
Interleukin-8 (IL-8) is a key mediator in the migration of neutrophils from the circulation to the site of inflammation in the tissue. IL-8 is secreted by many cell types in response to proinflammatory stimuli such as interleukin 1, tumor necrosis factor, and lipopolysaccharide and is a potent chemoattractant and activator of neutrophils. Neutrophil activating peptide-2 (NAP-2) and melanoma growth-stimulatory activity (MGSA/GRO) are structurally and functionally related to IL-8 and, like IL-8, bind to specific G protein-coupled receptors on neutrophils. In the present study two closely related cloned IL-8 receptor subtypes are characterized by expression of the cDNA clones in monkey kidney cells (COS-7) or chinese hamster ovary cells and analysis of their ligand binding profiles. Both receptor subtypes bind 125I-labeled IL-8 with similar high affinity, however, the F3R receptor binds IL-8 exclusively, while the 4Ab receptor binds both IL-8 and MGSA/GRO with high affinity and NAP-2 with lesser affinity. Furthermore, we demonstrate with the use of intersubtype chimeric receptors that the specificity of ligand binding to both IL-8 receptor subtypes is dictated by the heterogeneous NH2-terminal domain. The F3R receptor is representative of a restricted IL-8 receptor subtype, and 4Ab represents a nonrestricted receptor subtype. It is proposed that these subtypes be named IL-8 receptors alpha and beta, respectively.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8A,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
267
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
25402-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1281158-Amino Acid Sequence,
pubmed-meshheading:1281158-Animals,
pubmed-meshheading:1281158-Binding, Competitive,
pubmed-meshheading:1281158-Cell Line,
pubmed-meshheading:1281158-Cloning, Molecular,
pubmed-meshheading:1281158-DNA,
pubmed-meshheading:1281158-Gene Library,
pubmed-meshheading:1281158-Humans,
pubmed-meshheading:1281158-Interleukin-8,
pubmed-meshheading:1281158-Kinetics,
pubmed-meshheading:1281158-Leukocytes,
pubmed-meshheading:1281158-Lymphocytes,
pubmed-meshheading:1281158-Models, Structural,
pubmed-meshheading:1281158-Molecular Sequence Data,
pubmed-meshheading:1281158-Neutrophils,
pubmed-meshheading:1281158-Organ Specificity,
pubmed-meshheading:1281158-Plasmids,
pubmed-meshheading:1281158-Protein Structure, Secondary,
pubmed-meshheading:1281158-RNA,
pubmed-meshheading:1281158-Receptors, Immunologic,
pubmed-meshheading:1281158-Receptors, Interleukin-8A,
pubmed-meshheading:1281158-Recombinant Fusion Proteins,
pubmed-meshheading:1281158-Transfection
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Amino terminus of the interleukin-8 receptor is a major determinant of receptor subtype specificity.
|
| pubmed:affiliation |
Repligen Corporation, Department of Molecular Biology, Cambridge, Massachusetts 02139.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|