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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
35
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pubmed:dateCreated |
1993-1-12
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pubmed:abstractText |
Using conditions that produced chronic inflammation in rat liver, we were able to find a correlation between induction of nitric oxide production and inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12). This enzyme is a tetramer composed of identical M(r) 37,000 subunits. The tetramer contains 16 thiol groups, four of which are essential for enzymatic activity. Our information indicates that four thiol groups are S-nitrosylated by exposure to authentic nitric oxide (NO) gas. Furthermore, NO decreased GAPDH activity while increasing its auto-ADP-ribosylation. Reduced nicotinamide adenine dinucleotide and dithiothreitol are required for the S-nitrosylation of GAPDH caused by the NO-generating compound sodium nitroprusside. Our results suggests that a new and important action of nitric oxide on cells is the S-nitrosylation and inactivation of GAPDH. S-Nitrosylation of GAPDH may be a key covalent modification of multiple regulatory consequences in chronic liver inflammation.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
24929-32
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1281150-Adenosine Diphosphate Ribose,
pubmed-meshheading:1281150-Amino Acid Oxidoreductases,
pubmed-meshheading:1281150-Animals,
pubmed-meshheading:1281150-Cytosol,
pubmed-meshheading:1281150-Dithionitrobenzoic Acid,
pubmed-meshheading:1281150-Dithiothreitol,
pubmed-meshheading:1281150-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:1281150-Gram-Positive Bacterial Infections,
pubmed-meshheading:1281150-Kinetics,
pubmed-meshheading:1281150-Liver,
pubmed-meshheading:1281150-Macromolecular Substances,
pubmed-meshheading:1281150-Male,
pubmed-meshheading:1281150-NAD,
pubmed-meshheading:1281150-Nitric Oxide,
pubmed-meshheading:1281150-Nitric Oxide Synthase,
pubmed-meshheading:1281150-Nitroso Compounds,
pubmed-meshheading:1281150-Oxidation-Reduction,
pubmed-meshheading:1281150-Propionibacterium acnes,
pubmed-meshheading:1281150-Rats,
pubmed-meshheading:1281150-Rats, Sprague-Dawley
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pubmed:year |
1992
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pubmed:articleTitle |
Nitric oxide-induced S-nitrosylation of glyceraldehyde-3-phosphate dehydrogenase inhibits enzymatic activity and increases endogenous ADP-ribosylation.
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pubmed:affiliation |
Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.
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pubmed:publicationType |
Journal Article
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