Source:http://linkedlifedata.com/resource/pubmed/id/12811453
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2003-7-15
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| pubmed:abstractText |
Bond Elut C(18) solid-phase extraction cartridges were used for pre-concentration followed by derivatization with o-phthaldialdehyde-N-acetylcysteine (OPA-NAC) of primary amines in water. Optimal conditions were: conditioning the cartridges with borate buffer pH 10.4, retention of the primary amines, addition of the OPA-NAC(3.7 mmol L(-1)) 1:1 molar ratio and borate buffer pH 8, elution of the isoindol with MeOH-borate buffer (9:1) pH 10.2 and fluorescence measurement. The equations of the calibration graphs for methylamine, ethylamine, propylamine, butylamine, pentylamine, and beta-phenylethylamine at lambda(excitation)=330 nm and lambda(emission)=440 nm, in the optimal conditions are presented. The solid-phase extraction procedure improved ten times the detection limits of the solution derivatization. Those values are in the 0.01-0.06 mg L(-1) interval in function of the amine. Also, it is possible to estimate the total primary aliphatic amine concentration in water, expressed as molar concentration of -NH(2) group or -NH(2)-N mg L(-1). On the basis of these studies, the method was applied for the determination of primary amino groups in tap, ground, factory and source water samples.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1618-2642
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
376
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
918-22
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| pubmed:year |
2003
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| pubmed:articleTitle |
Rapid fluorimetric assay for primary amine groups in water samples.
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| pubmed:affiliation |
Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
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| pubmed:publicationType |
Journal Article
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