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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2003-6-17
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pubmed:abstractText |
The universal genetic code links the 20 naturally occurring amino acids to the 61 sense codons. Previously, the UAG amber stop codon (a nonsense codon) has been used as a blank in the code to insert natural and unnatural amino acids via nonsense suppression. We have developed a selection methodology to investigate whether the unnatural amino acid biocytin could be incorporated into an mRNA display library at sense codons. In these experiments we probed a single randomized NNN codon with a library of 16 orthogonal, biocytin-acylated tRNAs. In vitro selection for efficient incorporation of the unnatural amino acid resulted in templates containing the GUA codon at the randomized position. This sense suppression occurs via Watson-Crick pairing with similar efficiency to UAG-mediated nonsense suppression. These experiments suggest that sense codon suppression is a viable means to expand the chemical and functional diversity of the genetic code.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-10359713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-10583945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-10668803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-10830162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11102869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11350040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11453067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11479568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11567528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11588263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-11675589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-12120285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-12188645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-12403461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-12464183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-12470735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-2677635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-5969078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-6345068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-6372858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-6656641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-8187878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-8798511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-9356443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-9801296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12810911-9848650
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1355-8382
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
780-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12810911-Amino Acid Sequence,
pubmed-meshheading:12810911-Base Sequence,
pubmed-meshheading:12810911-Codon,
pubmed-meshheading:12810911-DNA, Complementary,
pubmed-meshheading:12810911-Genetic Code,
pubmed-meshheading:12810911-Molecular Sequence Data,
pubmed-meshheading:12810911-Oligodeoxyribonucleotides,
pubmed-meshheading:12810911-Plasmids,
pubmed-meshheading:12810911-RNA, Antisense,
pubmed-meshheading:12810911-RNA, Messenger,
pubmed-meshheading:12810911-RNA, Transfer,
pubmed-meshheading:12810911-Templates, Genetic
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pubmed:year |
2003
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pubmed:articleTitle |
In vitro selection for sense codon suppression.
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pubmed:affiliation |
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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