Source:http://linkedlifedata.com/resource/pubmed/id/12810350
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2003-6-17
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| pubmed:abstractText |
Apoptosis plays an important role in immune responses, but little is known about its involvement in contact hypersensitivity (CH). In this study, we have investigated the role of Fas/Fas ligand (FasL)-mediated apoptosis in the pathogenesis of CH. Mice were sensitized by one topical application of 100 microl of 3% oxazolone to shaved skin of the abdomen. Six days later, CH was provoked by challenging both sides of sensitized mouse right ear with 15 microl of 1% oxazolone. Using a DNA ladder assay, we found that apoptosis was induced in the skin of oxazolone-sensitized mice 24-96 h after allergen challenge. Annexin V-fluorescein isothiocyanate (FITC)-propidium iodide (PI) apoptosis flow cytometric assay showed that early apoptotic CD4(+) T cells (annexin V-FITC(+)PI(-)), but not late apoptotic CD4(+) T cells (annexin V-FITC(+)PI(+)), increased in the inflamed skin of mice with CH. Moreover, the expressions of mRNAs for T helper (Th2) cytokine (interleukin (IL)-4), Th1 cytokine (interferon (IFN)-gamma) and proapoptotic molecules (Bax, Fas, FasL and IL-1beta-converting enzyme (ICE)/caspase-1) were significantly elevated in the oxazolone-sensitized mouse skin 6-72 h after allergen challenge. Dramatic increase in IL-10 mRNA was only observed in the sensitized mouse skin 6 and 12 h after allergen challenge. Furthermore, CH was significantly inhibited with decreased apoptosis and early apoptotic CD4(+) T cells in inflamed skin in Fas mutant lpr/lpr mice compared to wild-type mice, whereas there were no significant differences in IL-4, IFN-gamma, IL-10, Bax and ICE mRNAs in the inflamed skin of CH between lpr/lpr and wild-type mice. Our results thus suggest that Fas/FasL pathway partially contributes to apoptosis in murine CH and that Fas/FasL-mediated apoptosis plays a partial role in the development of CH. The contribution of Fas/FasL-mediated apoptosis to CH appears independent of Th1 and Th2 cytokines.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Bax protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Fasl protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oxazolone,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1567-5769
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
3
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
927-38
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12810350-Adjuvants, Immunologic,
pubmed-meshheading:12810350-Administration, Cutaneous,
pubmed-meshheading:12810350-Animals,
pubmed-meshheading:12810350-Antigens, CD95,
pubmed-meshheading:12810350-Apoptosis,
pubmed-meshheading:12810350-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12810350-Caspase 1,
pubmed-meshheading:12810350-DNA Fragmentation,
pubmed-meshheading:12810350-Dermatitis, Contact,
pubmed-meshheading:12810350-Fas Ligand Protein,
pubmed-meshheading:12810350-Female,
pubmed-meshheading:12810350-Interferon-gamma,
pubmed-meshheading:12810350-Interleukin-10,
pubmed-meshheading:12810350-Interleukin-4,
pubmed-meshheading:12810350-Membrane Glycoproteins,
pubmed-meshheading:12810350-Mice,
pubmed-meshheading:12810350-Mice, Inbred BALB C,
pubmed-meshheading:12810350-Mice, Inbred C57BL,
pubmed-meshheading:12810350-Mice, Mutant Strains,
pubmed-meshheading:12810350-Oxazolone,
pubmed-meshheading:12810350-Proto-Oncogene Proteins,
pubmed-meshheading:12810350-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:12810350-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12810350-bcl-2-Associated X Protein
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| pubmed:year |
2003
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| pubmed:articleTitle |
Role of Fas/Fas ligand-mediated apoptosis in murine contact hypersensitivity.
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| pubmed:affiliation |
Department of Environmental Medicine, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan. xubaohui@m.kufm.kagoshima-u.ac.jp
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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