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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-6-17
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pubmed:abstractText |
In this study we used in situ hybridisation and double-labelling immunohistochemistry to characterise cannabinoid receptor 1 (CB(1)) expression in rat lumbar dorsal root ganglion (DRG) neurons.Approximately 25% of DRG neurons expressed CB(1) mRNA and displayed immunoreactivity for CB(1). Sixty-nine percent to 82% of CB(1)-expressing cells were also immunoreactive for neurofilament 200, indicative of myelinated A-fibre neurons, which tend to be large- and medium-sized DRG neurons (>600 microm(2)). Approximately 10% of CB1-expressing cells also expressed transient receptor potential vanilloid family ion channel 2 (TRPV2), the noxious heat-transducing channel found in medium to large lightly myelinated Adelta-fibre DRG neurons. Seventeen percent to 26% of CB(1)-expressing cells co-stained using Isolectin B4, 9-10% for calcitonin gene-related peptide and 11-20% for transient receptor potential vanilloid family ion channel 1 (TRPV1), predominantly markers of small non-myelinated C-fibre DRG neurons (<600 microm(2)). These findings suggest that whilst a wide range of DRG neuron phenotypes express CB(1), it is predominantly associated with myelinated fibres.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin Gene-Related Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurofilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cannabinoid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/isolectin B4-binding glycoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/neurofilament protein H
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pubmed:status |
MEDLINE
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pubmed:issn |
0306-4522
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
119
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
803-12
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pubmed:dateRevised |
2009-11-3
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pubmed:meshHeading |
pubmed-meshheading:12809701-Animals,
pubmed-meshheading:12809701-Calcitonin Gene-Related Peptide,
pubmed-meshheading:12809701-Cell Size,
pubmed-meshheading:12809701-Fluorescent Antibody Technique,
pubmed-meshheading:12809701-Ganglia, Spinal,
pubmed-meshheading:12809701-Glycoproteins,
pubmed-meshheading:12809701-Lectins,
pubmed-meshheading:12809701-Male,
pubmed-meshheading:12809701-Mechanoreceptors,
pubmed-meshheading:12809701-Mice,
pubmed-meshheading:12809701-Mice, Knockout,
pubmed-meshheading:12809701-Nerve Fibers, Myelinated,
pubmed-meshheading:12809701-Nerve Fibers, Unmyelinated,
pubmed-meshheading:12809701-Neurofilament Proteins,
pubmed-meshheading:12809701-Neurons, Afferent,
pubmed-meshheading:12809701-Nociceptors,
pubmed-meshheading:12809701-Pain,
pubmed-meshheading:12809701-RNA, Messenger,
pubmed-meshheading:12809701-Rats,
pubmed-meshheading:12809701-Rats, Wistar,
pubmed-meshheading:12809701-Receptors, Cannabinoid,
pubmed-meshheading:12809701-Receptors, Drug
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pubmed:year |
2003
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pubmed:articleTitle |
Localisation of cannabinoid receptor 1 in rat dorsal root ganglion using in situ hybridisation and immunohistochemistry.
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pubmed:affiliation |
Pain Research, Department of Anaesthetics, Faculty of Medicine, Imperial College, Chelsea and Westminster Hospital Campus, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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