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rdf:type |
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lifeskim:mentions |
umls-concept:C0040648,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0205107,
umls-concept:C0212320,
umls-concept:C0441655,
umls-concept:C0526260,
umls-concept:C0851285,
umls-concept:C1333079,
umls-concept:C1413570,
umls-concept:C1428360,
umls-concept:C1979844
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pubmed:issue |
35
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pubmed:dateCreated |
2003-8-25
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pubmed:abstractText |
We have characterized the proximal promoter region of the human COL11A1 gene. Transient transfection assays indicate that the segment from -199 to +1 is necessary for the activation of basal transcription. Electrophoretic mobility shift assays (EMSAs) demonstrated that the ATTGG sequence, within the -147 to -121 fragment, is critical to bind nuclear proteins in the proximal COL11A1 promoter. We demonstrated that the CCAAT binding factor (CBF/NF-Y) bound to this region using an interference assay with consensus oligonucleotides and a supershift assay with specific antibodies in an EMSA. In a chromatin immunoprecipitation assay and EMSA using DNA-affinity-purified proteins, CBF/NF-Y proteins directly bound this region in vitro and in vivo. We also showed that four tandem copies of the CBF/NF-Y-binding fragment produced higher transcriptional activity than one or two copies, whereas the absence of a CBF/NF-Y-binding fragment suppressed the COL11A1 promoter activity. Furthermore, overexpression of a dominant-negative CBF-B/NF-YA subunit significantly inhibited promoter activity in both transient and stable cells. These results indicate that the CBF/NF-Y proteins regulate the transcription of COL11A1 by directly binding to the ATTGG sequence in the proximal promoter region.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
32763-70
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12805369-Base Sequence,
pubmed-meshheading:12805369-Binding Sites,
pubmed-meshheading:12805369-CCAAT-Binding Factor,
pubmed-meshheading:12805369-Cell Nucleus,
pubmed-meshheading:12805369-Chromatin,
pubmed-meshheading:12805369-Collagen,
pubmed-meshheading:12805369-Collagen Type XI,
pubmed-meshheading:12805369-DNA,
pubmed-meshheading:12805369-Gene Deletion,
pubmed-meshheading:12805369-Gene Expression Regulation,
pubmed-meshheading:12805369-Genes, Dominant,
pubmed-meshheading:12805369-Humans,
pubmed-meshheading:12805369-Luciferases,
pubmed-meshheading:12805369-Models, Genetic,
pubmed-meshheading:12805369-Molecular Sequence Data,
pubmed-meshheading:12805369-Plasmids,
pubmed-meshheading:12805369-Precipitin Tests,
pubmed-meshheading:12805369-Promoter Regions, Genetic,
pubmed-meshheading:12805369-Protein Binding,
pubmed-meshheading:12805369-Recombinant Fusion Proteins,
pubmed-meshheading:12805369-Transcription, Genetic,
pubmed-meshheading:12805369-Transcription Factors,
pubmed-meshheading:12805369-Transfection,
pubmed-meshheading:12805369-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
The transcription factor CCAAT-binding factor CBF/NF-Y regulates the proximal promoter activity in the human alpha 1(XI) collagen gene (COL11A1).
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pubmed:affiliation |
Department of Anatomy, Biology, and Medicine, Oita Medical University, Hasama-machi, Oita 879-5593, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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