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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-6-13
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pubmed:abstractText |
Spermidine/spermine N(1)-acetyltransferase (SSAT) regulates polyamine catabolism. Thioredoxin-1 (Trx-1) is a redox protein that is overexpressed in human cancer leading to increased cell proliferation, decreased apoptosis, and decreased patient survival. We report that SSAT mRNA expression is decreased in Trx-1 transfected MCF-7 human breast cancer cells. There is also a decrease in SSAT enzyme activity and lower putrescine levels but no change in spermine or spermidine levels. The expression of SSAT is regulated by the NF-E2-related factor 2 (Nrf-2) and polyamine modulated factor-1 (PMF-1) transcription factor complex. Trx-1 transfected MCF-7 cells showed decreased Nrf-2/PMF-1 DNA binding without a change in Nrf-2 or PMF-1 protein expression. The results suggest that Trx-1 may play a role in the redox regulation of SSAT expression and polyamine homeostasis that could contribute to the biological effects of Trx-1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2-Related Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/NFE2L2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PMF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Polyamines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TXN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Thioredoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/diamine N-acetyltransferase
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
306
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
469-75
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12804587-Acetyltransferases,
pubmed-meshheading:12804587-Apoptosis,
pubmed-meshheading:12804587-Blotting, Northern,
pubmed-meshheading:12804587-Blotting, Western,
pubmed-meshheading:12804587-Breast Neoplasms,
pubmed-meshheading:12804587-Cell Division,
pubmed-meshheading:12804587-Cell Nucleus,
pubmed-meshheading:12804587-DNA,
pubmed-meshheading:12804587-DNA-Binding Proteins,
pubmed-meshheading:12804587-Humans,
pubmed-meshheading:12804587-Membrane Proteins,
pubmed-meshheading:12804587-NF-E2-Related Factor 2,
pubmed-meshheading:12804587-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12804587-Oxidation-Reduction,
pubmed-meshheading:12804587-Polyamines,
pubmed-meshheading:12804587-Protein Binding,
pubmed-meshheading:12804587-RNA, Messenger,
pubmed-meshheading:12804587-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12804587-Thioredoxins,
pubmed-meshheading:12804587-Trans-Activators,
pubmed-meshheading:12804587-Transcription Factors,
pubmed-meshheading:12804587-Transfection,
pubmed-meshheading:12804587-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Increased thioredoxin-1 inhibits SSAT expression in MCF-7 human breast cancer cells.
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pubmed:affiliation |
Arizona Cancer Center, University of Arizona, Tucson, AZ 85724-5024, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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