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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-6-13
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pubmed:abstractText |
Coculture of mouse bone marrow-derived mast cells (BMMC) with fibroblasts in the presence of stem cell factor (SCF) facilitates morphological and functional maturation toward a connective tissue mast cell (CTMC)-like phenotype. By means of cDNA subtraction, we identified several inducible genes during this mast cell maturation process. Of approximately 100 sequenced clones induced, nearly 50% were chromosome 14-associated serine proteases. Approximately 14% encoded NDRG1, a 43-kDa cytosolic protein that has been implicated in cell differentiation. NDRG1 was distributed in the cytosol of cultured mast cells and CTMC in rat skin. Overexpression of NDRG1 in RBL-2H3 cells resulted in enhanced degranulation in response to various stimuli. Thus, NDRG1 may be a mast cell maturation-associated inducible protein that allows the cells to be susceptible to extracellular stimuli leading to degranulation. Additionally, several unique maturation-associated inducible genes were identified, molecular and functional characterization of which will provide new insights into mast cell biology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:ChangHyeun WookHW,
pubmed-author:HisadaMasatoM,
pubmed-author:IshiiShin ichiS,
pubmed-author:Kikuchi-YanoshitaReiR,
pubmed-author:KudoIchiroI,
pubmed-author:MiyazawaShinobuS,
pubmed-author:MoonTae ChulTC,
pubmed-author:MurakamiMakotoM,
pubmed-author:NatoriYasuhiroY,
pubmed-author:SaitoTakanoriT,
pubmed-author:SugikiToshihikoT,
pubmed-author:Suzuki-NishimuraTamikoT,
pubmed-author:TaketomiYoshitakaY,
pubmed-author:UchidaMasaatsu KMK
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
306
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-46
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12804568-Amino Acid Sequence,
pubmed-meshheading:12804568-Animals,
pubmed-meshheading:12804568-Cell Cycle Proteins,
pubmed-meshheading:12804568-Cell Line,
pubmed-meshheading:12804568-Cells, Cultured,
pubmed-meshheading:12804568-Coculture Techniques,
pubmed-meshheading:12804568-Cytosol,
pubmed-meshheading:12804568-DNA, Complementary,
pubmed-meshheading:12804568-Dose-Response Relationship, Drug,
pubmed-meshheading:12804568-Fibroblasts,
pubmed-meshheading:12804568-Immunohistochemistry,
pubmed-meshheading:12804568-Inflammation,
pubmed-meshheading:12804568-Insects,
pubmed-meshheading:12804568-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12804568-Ligands,
pubmed-meshheading:12804568-Mast Cells,
pubmed-meshheading:12804568-Mice,
pubmed-meshheading:12804568-Mice, Inbred BALB C,
pubmed-meshheading:12804568-Microscopy, Confocal,
pubmed-meshheading:12804568-Models, Genetic,
pubmed-meshheading:12804568-Molecular Sequence Data,
pubmed-meshheading:12804568-Mutation,
pubmed-meshheading:12804568-Phenotype,
pubmed-meshheading:12804568-Polymerase Chain Reaction,
pubmed-meshheading:12804568-Protein Structure, Tertiary,
pubmed-meshheading:12804568-Rats,
pubmed-meshheading:12804568-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
Identification of NDRG1 as an early inducible gene during in vitro maturation of cultured mast cells.
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pubmed:affiliation |
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, 142-8555, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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