Source:http://linkedlifedata.com/resource/pubmed/id/12804532
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2003-6-13
|
| pubmed:abstractText |
Terminal deoxynucleotidyl transferase (TdT) places non-template-coded nucleotides (N additions) in the VH CDR3 of T cell receptors and immunoglobulins. Amino acids coded for by N additions are important in autoantibody binding of dsDNA in lupus. We hypothesized that a genetic lack of TdT would modulate disease in lupus-prone mice. To test this hypothesis, we derived TdT-deficient MRL/lpr mice. Serum levels of anti-dsDNA antibodies and anti-dsDNA producing splenocytes were significantly lower in the TdT(-) versus TdT(+) littermates. Albuminuria, glomerular IgG deposition, and pathologic renal disease were significantly reduced in the TdT(-) mice. Sequence analysis of anti-dsDNA hybridomas derived from TdT(-) mice revealed a lack of N additions, short VH CDR3 segments, yet the presence of VH CDR3 arginines. Thus, the genetic absence of TdT reduces autoantibody production and clinical disease in MRL/lpr mice, confirming the importance of N additions in the autoimmune response in these mice.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidylexotransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1521-6616
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
107
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
186-97
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12804532-Albuminuria,
pubmed-meshheading:12804532-Animals,
pubmed-meshheading:12804532-Antibodies,
pubmed-meshheading:12804532-Autoantibodies,
pubmed-meshheading:12804532-B-Lymphocytes,
pubmed-meshheading:12804532-Base Sequence,
pubmed-meshheading:12804532-Biological Markers,
pubmed-meshheading:12804532-DNA Nucleotidylexotransferase,
pubmed-meshheading:12804532-Glomerulonephritis,
pubmed-meshheading:12804532-Immunoglobulin G,
pubmed-meshheading:12804532-Joint Diseases,
pubmed-meshheading:12804532-Joints,
pubmed-meshheading:12804532-Kidney,
pubmed-meshheading:12804532-Kidney Diseases,
pubmed-meshheading:12804532-Lung,
pubmed-meshheading:12804532-Lung Diseases,
pubmed-meshheading:12804532-Mice,
pubmed-meshheading:12804532-Mice, Inbred MRL lpr,
pubmed-meshheading:12804532-T-Lymphocytes
|
| pubmed:year |
2003
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| pubmed:articleTitle |
Effect of genetic deficiency of terminal deoxynucleotidyl transferase on autoantibody production and renal disease in MRL/lpr mice.
|
| pubmed:affiliation |
Medical Research Service, Ralph H. Johnson VAMC, and the Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA. gilkeson@musc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|