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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1992-12-29
pubmed:abstractText
We have established the human nck sequence as a new oncogene. Nck encodes one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase-activating protein, phosphatidylinositol 3-kinase, and phospholipase C-gamma. Overexpression of human nck in 3Y1 rat fibroblasts results in transformation as judged by alteration of cell morphology, colony formation in soft agar, and tumor formation in nude BALB/c mice. However, overexpression of nck does not induce detectable elevation of the phosphotyrosine content of specific proteins, as is observed for v-crk, another SH2/SH3-containing oncogene. Despite this fact, we demonstrate that Nck retains the ability to bind tyrosine phosphorylated proteins in vitro, using a fusion protein of Nck with glutathione-S-transferase (GST). Moreover, when incubated with lysates prepared from v-src-transformed 3Y1 cells or the nck-overexpressing cell lines, GST-Nck binds to both p60v-src and serine/threonine kinases, respectively. Although phosphotyrosine levels are not elevated in the nck-expressing fibroblasts, vanadate treatment of these cells results in a phosphotyrosine pattern that is altered from the parental 3Y1 pattern, suggestive of a perturbation of indigenous tyrosine kinase pathways. These results suggest the possibility that human nck induces transformation in 3Y1 fibroblasts by virtue of its altered affinity or specificity for the normal substrates of its rat homolog and that Nck may play a role in linking tyrosine and serine/threonine kinase pathways within the cell.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1312663, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1329926, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1333046, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1333047, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1372395, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1448108, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1691440, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1692963, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1695251, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1696179, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1700434, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1705010, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1707345, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1708916, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1710979, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1849460, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1849461, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-1979659, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2069873, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2107526, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2156626, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2157284, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2160078, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2176151, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2190221, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2236073, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2450282, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2463469, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2472218, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2496972, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2542016, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2831461, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2840660, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-2855503, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-3023673, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-3025655, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-3047011, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-3201259, http://linkedlifedata.com/resource/pubmed/commentcorrection/1280326-6356358
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:geneSymbol
nck
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5834-42
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed-meshheading:1280326-Humans, pubmed-meshheading:1280326-Animals, pubmed-meshheading:1280326-Mice, pubmed-meshheading:1280326-Sulfhydryl Compounds, pubmed-meshheading:1280326-Rats, pubmed-meshheading:1280326-Tyrosine, pubmed-meshheading:1280326-Cell Transformation, Neoplastic, pubmed-meshheading:1280326-Fibroblasts, pubmed-meshheading:1280326-Cell Line, pubmed-meshheading:1280326-Mice, Inbred BALB C, pubmed-meshheading:1280326-Ligands, pubmed-meshheading:1280326-Vanadates, pubmed-meshheading:1280326-Phosphotyrosine, pubmed-meshheading:1280326-Oncogene Protein pp60(v-src), pubmed-meshheading:1280326-Retroviridae Proteins, Oncogenic, pubmed-meshheading:1280326-Immunoblotting, pubmed-meshheading:1280326-Adaptor Proteins, Signal Transducing, pubmed-meshheading:1280326-Oncogene Proteins, pubmed-meshheading:1280326-Oncogene Protein v-crk
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