Linked Life Data

1280266

Source:http://linkedlifedata.com/resource/pubmed/id/1280266

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
1992-12-30
pubmed:abstractText
We have previously reported an anti-fibronectin monoclonal antibody (mAb) (BC-1) which reacts with an ED-B-containing beta-galactosidase-fibronectin fusion protein but not with an identical beta-galactosidase-fibronectin fusion protein in which the ED-B sequence is omitted. In further experiments aimed at localizing more precisely the epitope recognized by this mAb, we demonstrate that 1) the mAb BC-1 is indeed specific for ED-B-containing fibronectin (FN) molecules even though the epitope recognized by this mAb is localized on the type III homology repeat 7 (the one which precedes the ED-B sequence) and 2) in fibronectin molecules lacking the ED-B sequence, this epitope is masked. We further demonstrate that, to mask the epitope recognized by the mAb BC-1, the presence of at least half of the FN type III homology repeat 9 is necessary. We also report the production of the mAb IST-6 which recognizes only FN molecules in which the ED-B sequence is lacking. These data clearly demonstrate that the presence of the ED-B sequence within FN molecules generates conformational modification in the central part of the molecules that unmasks previously cryptic sequences and masks others.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24689-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1280266-Amino Acid Sequence, pubmed-meshheading:1280266-Antibodies, Monoclonal, pubmed-meshheading:1280266-Cell Line, pubmed-meshheading:1280266-Cell Line, Transformed, pubmed-meshheading:1280266-Cloning, Molecular, pubmed-meshheading:1280266-Epitopes, pubmed-meshheading:1280266-Escherichia coli, pubmed-meshheading:1280266-Fibronectins, pubmed-meshheading:1280266-Humans, pubmed-meshheading:1280266-Immunoenzyme Techniques, pubmed-meshheading:1280266-Macromolecular Substances, pubmed-meshheading:1280266-Protein Conformation, pubmed-meshheading:1280266-Radioimmunoassay, pubmed-meshheading:1280266-Recombinant Fusion Proteins, pubmed-meshheading:1280266-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:1280266-Sequence Homology, Amino Acid, pubmed-meshheading:1280266-beta-Galactosidase
pubmed:year
1992
pubmed:articleTitle
The inclusion of the type III repeat ED-B in the fibronectin molecule generates conformational modifications that unmask a cryptic sequence.
pubmed:affiliation
Laboratory of Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't