Linked Life Data

12801509

Source:http://linkedlifedata.com/resource/pubmed/id/12801509

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-12
pubmed:databankReference
pubmed:abstractText
Plant nonspecific lipid transfer proteins (nsLTPs) are characterized by their ability to bind a broad range of hydrophobic ligands in vitro. Their biological function has not yet been elucidated, but they could play a major role in plant defense to physical and biological stress. An nsLTP was isolated from Amaranthus hypochondriacus seeds and purified by gel filtration and reversed-phase high-performance liquid chromatography techniques. The molecular mass of the protein as determined by mass spectrometry is 9747.29 Da. Data from amino acid sequence, circular dichroism and binding/displacement of a fluorescent lipid revealed that it belongs to the nsLTP1 family. The protein shows the alpha-helical secondary structure typical for plant nsLTPs 1 and shares 40 to 57% sequence identity with nsLTPs 1 from other plant species and 100% identity with an nsLTP1 from Amaranthus caudatus. A model structure of the protein in complex with stearate based on known structures of maize and rice nsLTPs 1 suggests a protein fold complexed with lipids closely related to that of maize nsLTP1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
415
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12801509-Amaranthus, pubmed-meshheading:12801509-Amino Acid Sequence, pubmed-meshheading:12801509-Antigens, Plant, pubmed-meshheading:12801509-Carrier Proteins, pubmed-meshheading:12801509-Chromatography, High Pressure Liquid, pubmed-meshheading:12801509-Circular Dichroism, pubmed-meshheading:12801509-Lipids, pubmed-meshheading:12801509-Models, Molecular, pubmed-meshheading:12801509-Molecular Sequence Data, pubmed-meshheading:12801509-Molecular Weight, pubmed-meshheading:12801509-Plant Proteins, pubmed-meshheading:12801509-Protein Binding, pubmed-meshheading:12801509-Protein Structure, Secondary, pubmed-meshheading:12801509-Sequence Analysis, Protein, pubmed-meshheading:12801509-Species Specificity, pubmed-meshheading:12801509-Spectrometry, Mass, Matrix-Assisted Laser...
pubmed:year
2003
pubmed:articleTitle
Amino acid sequence, biochemical characterization, and comparative modeling of a nonspecific lipid transfer protein from Amaranthus hypochondriacus.
pubmed:affiliation
Departamento Sistemas Biológicos, UAM-X, 04960 México, D.F., Mexico.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't