Linked Life Data

12800198

Source:http://linkedlifedata.com/resource/pubmed/id/12800198

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-6-11
pubmed:abstractText
To design a specific immunotherapy for leukemia patients, the identification of leukemia-associated antigens (LAAs) is a pivotal step. Antileukemic effects after hematopoetic stem cell transplantation for myeloid leukemias are observed and might be related to the recognition of LAAs. Using the serological screening of an expression library (SEREX) of K562 cells, we identified 16 different clones encoding LAAs eliciting a humoral immune response, among them the heat shock proteins HSJ2 and HSP70, the M-phase phosphoprotein 11 (MPP11), the BRCA1-associated protein (BRAP), the Jkappa recombination binding protein (RBPJkappa) and the receptor for hyaluronic acid mediated motility (RHAMM). Serological responses to MPP11 were observed in 7/19 (37%) of patients with acute myeloid leukemia (AML) and 6/16 (38%) of patients with chronic myeloid leukemia (CML), but not in healthy volunteers (0/20). IgG antibodies directed against MPP11 were also detected in 25-50% of the sera of patients with solid tumors such as melanoma, renal cell, ovarian and breast carcinoma. mRNA expression of MPP11 was detected in 20/20 AML patients and 7/10 patients with CML. In normal tissues, strong mRNA expression of MPP11 was only detected in testis. By real-time PCR, we detected upregulation of MPP11 in leukemic blasts. Simultaneous humoral immune responses to 2 or more of the 16 LAAs identified here was observed, suggesting the feasibility of a polyvalent vaccination as an option for immunotherapies in leukemia patients.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
224-31
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed-meshheading:12800198-Acute Disease, pubmed-meshheading:12800198-Antibodies, Neoplasm, pubmed-meshheading:12800198-Antigens, Neoplasm, pubmed-meshheading:12800198-Breast Neoplasms, pubmed-meshheading:12800198-Carcinoma, Renal Cell, pubmed-meshheading:12800198-DNA-Binding Proteins, pubmed-meshheading:12800198-Female, pubmed-meshheading:12800198-Gene Expression, pubmed-meshheading:12800198-Humans, pubmed-meshheading:12800198-K562 Cells, pubmed-meshheading:12800198-Kidney Neoplasms, pubmed-meshheading:12800198-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:12800198-Neoplasm Proteins, pubmed-meshheading:12800198-Oncogene Proteins, pubmed-meshheading:12800198-RNA, Messenger, pubmed-meshheading:12800198-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12800198-Skin Neoplasms
pubmed:year
2003
pubmed:articleTitle
Characterization of several leukemia-associated antigens inducing humoral immune responses in acute and chronic myeloid leukemia.
pubmed:affiliation
Third Department of Internal Medicine, University of Ulm, Ulm, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't