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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5 Pt 2
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pubmed:dateCreated |
1992-12-22
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pubmed:abstractText |
We have recently isolated cDNAs encoding a Na(+)-H+ exchanger isoform, referred to as NHE-1, from rabbit kidney and LLC-PK1 cells. To identify the NHE-1 protein and to establish its cellular and subcellular localization in the rabbit kidney, we prepared antibodies to a NHE-1 fusion protein. cDNA encoding the COOH-terminal 41 amino acids of NHE-1 was subcloned into a maltose-binding protein vector and the purified fusion protein (FP347A) used to immunize guinea pigs. To identify the NHE-1 protein, we performed Western blot analysis against membrane fractions prepared from rabbit renal cortex. Anti-FP347A antibody specifically reacted with a polypeptide with an apparent molecular mass of 100-110 kDa that was enriched in basolateral membrane fractions. When indirect immunofluorescence was performed on semithin (0.5 micron) cryosections of paraformaldehyde-lysine-periodate-fixed rabbit kidney, anti-FP347A specifically stained the basolateral plasma membrane of cells of the proximal tubule, thick ascending limb, and distal convoluted tubule. Anti-FP347A similarly stained connecting tubule cells and principal cells. No staining was detected on the apical membrane of any cells of the rabbit nephron. We conclude that NHE-1 is a 100- to 110-kDa protein expressed on the basolateral membrane of multiple nephron segments.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/growth factor-activatable Na-H...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F833-40
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1279986-Animals,
pubmed-meshheading:1279986-Carrier Proteins,
pubmed-meshheading:1279986-Epithelial Cells,
pubmed-meshheading:1279986-Epithelium,
pubmed-meshheading:1279986-Immunoblotting,
pubmed-meshheading:1279986-Immunohistochemistry,
pubmed-meshheading:1279986-Kidney,
pubmed-meshheading:1279986-Kidney Tubules,
pubmed-meshheading:1279986-Membrane Proteins,
pubmed-meshheading:1279986-Nephrons,
pubmed-meshheading:1279986-Rabbits,
pubmed-meshheading:1279986-Sodium-Hydrogen Antiporter,
pubmed-meshheading:1279986-Staining and Labeling,
pubmed-meshheading:1279986-Tissue Distribution
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pubmed:year |
1992
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pubmed:articleTitle |
Immunocytochemical characterization of Na(+)-H+ exchanger isoform NHE-1 in rabbit kidney.
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pubmed:affiliation |
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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