Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2003-6-11
pubmed:abstractText
Antigenes, which are substances that inhibit gene expression by binding to double-stranded DNA (dsDNA) in a sequence-specific manner, are currently sought for the treatment of various gene-related diseases. As such antigenes, we developed new nuclease-resistant oligopyrimidine nucleotides that are partially modified with 2'-O,4'-C-ethylene nucleic acids (ENA), which are constrained in the C3'-endo conformation and can form a triplex with dsDNA at physiological pH. It was found that these oligonucleotides formed triplexes similarly to those partially modified with 2'-O,4'-C-methylene nucleic acids (2',4'-BNA or LNA), as determined by UV melting analyses, electromobility shift assays, CD spectral analyses and restriction enzyme inhibition assays. In our studies, oligonucleotides fully modified with ENA have delta torsion angle values that are marginally higher than those of 2',4'-BNA/LNA. ENA oligonucleotides present in 10-fold the amount of dsDNA were found to be favorable in forming triplexes. These results provide useful information for the future design of triplex-forming oligonucleotides fully modified with such nucleic acids constrained in the C3'-endo conformation considering that oligonucleotides fully modified with 2',4'-BNA/LNA do not form triplexes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-10747893, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-10805816, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-10806994, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-10932156, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11035027, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11135550, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11170429, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11354656, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11738576, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11814825, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11972327, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-11978144, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-12713831, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-1741036, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-2041768, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-2251285, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-2359124, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-3118463, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-3671065, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-7525816, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-7541136, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-7906414, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-8662854, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-8943337, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-8978841, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-9358149, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-9459021, http://linkedlifedata.com/resource/pubmed/commentcorrection/12799454-9857031
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3267-73
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Triplex formation with 2'-O,4'-C-ethylene-bridged nucleic acids (ENA) having C3'-endo conformation at physiological pH.
pubmed:affiliation
Exploratory Chemistry Research Laboratories, Sankyo Co. Ltd, Tokyo 140-8710, Japan. koizum@shina.sankyo.co.jp
pubmed:publicationType
Journal Article, Comparative Study